AsPr John Stambas

STAFF PROFILE

Position

Associate Professor Of Medical Biology

Faculty

Faculty of Health

Department

School of Medicine

Campus

Geelong Waurn Ponds Campus

Qualifications

Bachelor of Science, University of Melbourne, 1996
Doctor of Philosophy, University of Melbourne, 2001
Graduate Certificate of Higher Education, Deakin University, 2012

Contact

john.stambas@deakin.edu.au
+61 3 522 72487

Research interests

Research in Dr Stambass laboratory is primarily focused on understanding immune responses following virus infection and vaccination. The main scientific aim is to carefully dissect immune responses following infection (influenza virus-including H5N1 and pandemic H1N1 2009) and vaccination (HIV vaccine candidates) to determine relevant immune correlates of protection in order to drive innovative strategies for future vaccine development. Dr Stambas has made a significant contribution to his field as evidenced by publication history (27 peer-reviewed articles to date) and his invitations to speak at national and international conferences.

Teaching interests

Dr Stambas serves as the Course Director for the Honours Program at the School of Medicine. Dr Stambas supervises honours and PhD students.

Units taught

HMM104, HMH 402, HMO 303

Awards

Dr Stambas has been awarded multiple research grants from local, national and international competitive schemes. These include three NHMRC grants as CI, an NHMRC Strategic Award for H1N1 Medical Research, an Australian Centre for HIV and Hepatitis Virology Research grant and a Bill and Melinda Gates Foundation Grand Challenges Exploration Grant. Dr Stambas has also received the highly competitive C.R. Roper Fellowship at the university of Melbourne and was awarded the Gordon Ada award from the Australasian Society for Immunology in 2012.

Publications

Filter by

2016

Recombinant influenza virus expressing HIV-1 p24 capsid protein induces mucosal HIV-specific CD8 T-cell responses

Hyon-Xhi Tan, Brad P. Gilbertson, Sinthujan Jegaskanda, Sheilajen Alcantara, Thakshila Amarasena, A/Prof John Stambas, Julie L. McAuley, Stephen Kent, Dr Robert De Rose

(2016), Vol. 34, pp. 1172-1179, Vaccine, Amsterdam, The Netherlands, C1

journal

ADAMTS5 is a critical regulator of virus-specific T cell immunity

Ms Meagan Mcmahon, Dr Siying Ye, Dr Leonard Izzard, Dr Daniel Dlugolenski, Dr Daniel Mcculloch, A/Prof John Stambas

(2016), Vol. 14, pp. 1-22, PLoS biology, San Francisco, Calif., C1

journal
2015

Inhibition of reactive oxygen species production ameliorates inflammation induced by influenza A viruses via upregulation of SOCS1 and SOCS3.

Dr Siying Ye, Sue Lowther, A/Prof John Stambas

(2015), Vol. 89, pp. 2672-2683, Journal of Virology, United States, C1

journal

Epitope-specific CD4+, but not CD8+, T-cell responses induced by recombinant influenza A viruses protect against Mycobacterium tuberculosis infection

Dr Manuela Florido, Roman Pillay, Caitlin Gillis, Ms Yingju Xia, Stephen J. Turner, Dr Jamie Triccas, A/Prof John Stambas, Prof Warwick Britton

(2015), Vol. 45, pp. 780-793, European journal of immunology, Weinheim, Germany, C1

journal

Molecular pathogenesis of H5 highly pathogenic avian influenza: the role of the haemagglutinin cleavage site motif

Jasmina Luczo, A/Prof John Stambas, Dr Peter Durr, Dr Wojtek Michalski, Dr John Bingham

(2015), Vol. 25, pp. 406-430, Reviews in medical virology, London, Eng., C1

journal
2014

Influenza reverse genetics: dissecting immunity and pathogenesis

Dr Siying Ye, Mr Justin Evans, A/Prof John Stambas

(2014), Vol. 16, pp. 1-19, Expert reviews in molecular medicine, Cambridge, Eng., C1

journal

miRNA modulation of SOCS1 using an influenza A virus delivery system

Dr Leonard Izzard, Dr Siying Ye, Kristie Jenkins, Ms Yingju Xia, Mark L. Tizard, A/Prof John Stambas

(2014), Vol. 95, pp. 1880-1885, Journal of general virology, Reading, England, C1

journal

Apocynin inhibits reactive oxygen species (ROS) and downregulates chemokine and cytokine production induced by avian influenza H5N1and H7N9 virus infection

A/Prof John Stambas, Dr Siying Ye

(2014), Vol. 192, pp. 1-1, Journal of immunology, Bethesda, Md., C1-1

journal
2013

Promotion of Hendra virus replication by microRNA 146a

Cameron R. Stewart, Dr Glenn Marsh, Kristie Jenkins, Michael P. Gantier, Mark L. Tizard, Dr Deborah Middleton, Dr John Lowenthal, Jessica Haining, Dr Leonard Izzard, Tamara Gough, Celine Deffrasnes, A/Prof John Stambas, Rachel Robinson, Hans G Heine, Jackie A Pallister, Adam J Foord, Andrew G.D Bean, Dr Linfa Wang

(2013), Vol. 87, pp. 3782-3791, Journal of virology, Washington, D. C., C1

journal

Influenza A virus infection impairs mycobacteria-specific T cell responses and mycobacterial clearance in the lung during pulmonary coinfection.

Dr Manuela Florido, M A Grima, C M Gillis, Ms Yingju Xia, A/Prof Stephen Turner, Dr Jamie Triccas, A/Prof John Stambas, Prof Warwick Britton

(2013), Vol. 191, pp. 302-311, Journal of immunology, Baltimore, Md., C1

journal

Glutathione peroxidase-1 reduces influenza A virus-induced lung inflammation

Selcuk Yatmaz, Huei Jiunn Seow, Rosa C Gualano, Zi Xin Wong, A/Prof John Stambas, Stavros Selemidis, Peter J. Crack, Steven Bozinovski, Gary Anderson, Ross Vlahos

(2013), Vol. 48, pp. 17-26, American journal of respiratory cell and molecular biology, New York, N.Y., C1

journal

Trivalent live attenuated influenza-simian immunodeficiency virus vaccines: efficacy and evolution of cytotoxic T lymphocyte escape in macaques.

Jeanette C. Reece, Sheilajen Alcantara, Shayarana Gooneratne, S Jegaskanda, Thakshila Amaresena, Caroline S. Fernandez, Karen Laurie, Aeron Hurt, Shelby L O'Connor, Max Harris, Dr Janka Petravic, Alexey Martyushev, Andrew J. Grimm, A/Prof Miles Davenport, A/Prof John Stambas, Dr Robert De Rose, Stephen J. Kent

(2013), Vol. 87, pp. 4146-4160, Journal of virology, Washington, D. C., C1

journal
2012

Comparison of influenza and SIV specific CD8 T cell responses in macaques

S Jegaskanda, Jeanette C. Reece, Dr Robert De Rose, A/Prof John Stambas, Lucy Sullivan, Andrew G. Brooks, Stephen J. Kent, Amy Sexton

(2012), Vol. 7, pp. 1-16, PLoS one, San Francisco, CA, C1-1

journal
2011

A gel-capture assay for characterizing the sialyl-glycan selectivity of influenza viruses

Dr Tony Velkov, Philip E Thompson, A/Prof Ossama El-Kabbani, F. Lindh, A/Prof John Stambas, S. Rockman

(2011), Vol. 55, pp. 131-137, Acta virologica, Bratislava, Slovakia, C1

journal

Inhibition of Nox2 oxidase activity ameliorates influenza A virus-induced lung inflammation

Ross Vlahos, A/Prof John Stambas, Steven Bozinovski, Bradley R. S. Broughton, Grant R. Drummond, Stavros Selemidis

(2011), Vol. 7, pp. 1-12, PLoS Pathogens, San Francisco, Calif., C1

journal

A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus–poxvirus prime boost immunisations

Charani Ranasinghe, Fiona Eyers, A/Prof John Stambas, Dr David Boyle, Ian A. Ramshaw, Alistair J. Ramsay

(2011), Vol. 29, pp. 3008-3020, Vaccine, Oxford, England, C1-1

journal
2010

Timing of immune escape linked to success or failure of vaccination

Jeanette C. Reece, Liyen Loh, Sheilajen Alcantara, Caroline S. Fernandez, A/Prof John Stambas, Amy Sexton, Dr Robert De Rose, Dr Janka Petravic, A/Prof Miles Davenport, Stephen J. Kent

(2010), Vol. 5, pp. 1-7, Plos one, San Francisco, Calif., C1-1

journal

Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

Katherine Kedzierska, Sophie A. Valkenburg, Carole Guillonneau, Francois-Xavier Hubert, Tania Cukalac, Joan M. Curtis, A/Prof John Stambas, Hamish S. Scott, Lukasz Kedzierski, Dr Vanessa Venturi, A/Prof Miles Davenport

(2010), Vol. 40, pp. 849-858, European journal of immunology, Weinheim, Germany, C1-1

journal

Influenza A virus-specific CD8.sup.+ T-cell responses : from induction to function

M. R. Olson, B. E. Russ, Peter C. Doherty, Stephen J. Turner, A/Prof John Stambas

(2010), Vol. 5, pp. 175-183, Future virology, London, England, C1

journal
2009

Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env 311-320 virus

Tania Cukalac, Jessica M. Moffat, Dr Vanessa Venturi, A/Prof Miles Davenport, Peter C. Doherty, Stephen J. Turner, A/Prof John Stambas

(2009), Vol. 27, pp. 6755-6761, Vaccine, Oxford, England, C1-1

journal

Evaluation of recombinant influenza virus-simian immunodeficiency virus vaccines in macaques

Amy Sexton, Dr Robert De Rose, Jeanette C. Reece, Sheilajen Alcantara, Liyen Loh, Jessica M. Moffat, Karen Laurie, Aeron Hurt, Peter C. Doherty, Stephen J. Turner, Stephen J. Kent, A/Prof John Stambas

(2009), Vol. 83, pp. 7619-7628, Journal of virology, Washington, DC, C1-1

journal
2008

Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity

Katherine Kedzierska, Nicole L. La Gruta, A/Prof John Stambas, Stephen J. Turner, Peter C. Doherty

(2008), Vol. 45, pp. 607-618, Molecular Immunology, Oxford, England, C1-1

journal

HIV-1 vaccine development : tackling virus diversity with a multi-envelope cocktail

J.L. Hurwitz, Xiaoyan Zhan, Scott A. Brown, Mattia Bonsignori, A/Prof John Stambas, T.D. Lockey, R. Sealy, S. Surman, P. Freiden, Bart Jones, Louis N. Martin, James L. Blanchard, K.S. Slobod

(2008), Vol. 13, pp. 609-620, Frontiers in Bioscience, Tampa, U.S.A., C1-1

journal

Killer T cells in influenza

A/Prof John Stambas, Carole Guillonneau, Katherine Kedzierska, Justine D Mintern, Peter C. Doherty, Nicole L. La Gruta

(2008), Vol. 120, pp. 186-196, Pharmacology and Therapeutics, Oxford, England, C1-1

journal

Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination

annett miller, Rob J. Center, A/Prof John Stambas, Georgia Deliyannis, Peter C. Doherty, Jane L. Howard, Stephen J. Turner, Damian F.J. Purcell

(2008), Vol. 26, pp. 5641-5648, Vaccine, Guildford, England, C1-1

journal
2007

A multi-vector, multi-envelope HIV-1 vaccine

J.L. Hurwitz, Xiaoyan Zhan, Scott A. Brown, Mattia Bonsignori, A/Prof John Stambas, T.D. Lockey, Bart Jones, S. Surman, R. Sealy, P. Freiden, Kristen Branum, K.S. Slobod

(2007), Vol. 12, pp. 68-76, The Journal of pediatric pharmacology and therapeutics, Littleton, Colorado, C1-1

journal

Location rather than CD62L phenotype is critical in the early establishment of influenza-specific T cell memory

Katherine Kedzierska, A/Prof John Stambas, Misty R. Jenkins, Rachael Keating, Stephen J. Turner, Peter C. Doherty

(2007), Vol. 104, pp. 9782-9787, Proceedings of the National Academy of Sciences of the United States of America, Washington, D.C., C1-1

journal

A question of self-preservation : immunopathology in influenza virus infection

Nicole L. La Gruta, Katherine Kedzierska, A/Prof John Stambas, Peter C. Doherty

(2007), Vol. 85, pp. 85-92, Immunology and Cell Biology, Adelaide, S. Aust., C1-1

journal

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells

A/Prof John Stambas, Peter C. Doherty, Stephen J. Turner

(2007), Vol. 178, pp. 1285-1292, The Journal of Immunology, Baltimore, Md., C1-1

journal
2005

Clade, country and region-specific HIV-1 vaccines : are they necessary?

K.S. Slobod, C. Coleclough, Scott A. Brown, A/Prof John Stambas, Xiaoyan Zhan, S. Surman, Bart Jones, A. Zirkel, P. Freiden, Brita Brown, R. Sealy, Mattia Bonsignori, J.L. Hurwitz

(2005), Vol. 2, pp. 1-3, AIDS Research and Therapy, London, England, C1-1

journal

Consequences of immunodominant epitope deletion for minor influenza virus-specific CD8+-T-cell responses

Samita S. Andreansky, A/Prof John Stambas, Paul G. Thomas, Weidong Xie, Richard J. Webby, Peter C. Doherty

(2005), Vol. 79, pp. 4329-4339, Journal of Virology, Washington, D.C., C1-1

journal

HIV vaccine rationale, design and testing

K.S. Slobod, C. Coleclough, Mattia Bonsignori, Scott A. Brown, Xiaoyan Zhan, S. Surman, A. Zirkel, Bart Jones, R. Sealy, A/Prof John Stambas, Brita Brown, T.D. Lockey, P. Freiden, Peter C. Doherty, James L. Blanchard, Louis N. Martin, J.L. Hurwitz

(2005), Vol. 3, pp. 107-112, Current HIV research, The Netherlands, C1-1

journal

HIV vaccines : brief review and discussion of future directions

K.S. Slobod, Mattia Bonsignori, Scott A. Brown, Xiaoyan Zhan, A/Prof John Stambas, J.L. Hurwitz

(2005), Vol. 4, pp. 305-313, Expert Review of Vaccines, London, U.K., C1-1

journal

Multi-envelope HIV-1 vaccine devoid of SIV components controls disease in macaques challenged with heterologous pathogenic SHIV

Xiaoyan Zhan, Louis N. Martin, K.S. Slobod, C. Coleclough, T.D. Lockey, Scott A. Brown, A/Prof John Stambas, Mattia Bonsignori, R. Sealy, James L. Blanchard, J.L. Hurwitz

(2005), Vol. 23, pp. 5306-5320, Vaccine, Guildford, England, C1-1

journal

Long lived multi-isotype anti-HIV antibody responses following a prime-double boost immunization strategy

A/Prof John Stambas, Scott A. Brown, A. Gutierrez, R. Sealy, W. Yue, Bart Jones, T.D. Lockey, A. Zirkel, P. Freiden, B. Brown, S. Surman, C. Coleclough, K.S. Slobod, Peter C. Doherty, J.L. Hurwitz

(2005), Vol. 23, pp. 2454-2464, Vaccine, Guildford, England, C1-1

journal
2004

Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

Samita S. Andreansky, A/Prof John Stambas, A. Gutierrez, Gabriela Diaz, Peter C. Doherty, Stephen J. Turner, Richard J. Webby

(2004), Vol. 1263, pp. 141-144, International Congress Series, The Netherlands, C1-1

journal

Differential tumor necrosis factor receptor 2-mediated editing of virus-specific CD8+ effector T cells

Stephen J. Turner, Nicole L. La Gruta, A/Prof John Stambas, Gabriela Diaz, Peter C. Doherty

(2004), Vol. 101, pp. 3545-3550, Proceedings of the National Academy of Sciences of the United States of America, Washington, D.C., C1-1

journal
2003

Protection and compensation in the influenza virus-specific CD8+ T cell response.

Richard J. Webby, Samita S. Andreansky, A/Prof John Stambas, Jerold E. Rehg, Robert G. Webster, Peter C. Doherty, Stephen J. Turner

(2003), Vol. 100, pp. 7235-7240, Proceedings of the National Academy of Sciences of the United States of America, Washington, D.C., C1-1

journal

Clustering of Th cell epitopes on exposed regions of HIV envelope despite defects in antibody activity

Scott A. Brown, A/Prof John Stambas, Xiaoyan Zhan, K.S. Slobod, C. Coleclough, A. Zirkel, S. Surman, Stephen W. White, Peter C. Doherty, J.L. Hurwitz

(2003), Vol. 171, pp. 4140-4148, The Journal of Immunology, Baltimore, Md., C1-1

journal
2002

Oxidised mannan-listeriolysin O conjugates induce Th1/Th2 cytokine responses after intranasal immunisation

A/Prof John Stambas, Geoffrey Pietersz, Ian McKenzie, Vijaya Nagabhushanam, Christina Cheers

(2002), Vol. 20, pp. 1877-1886, Vaccine, Guildford, England, C1-1

journal

Oxidised mannan as a novel adjuvant inducing mucosal IgA production.

A/Prof John Stambas, Geoffrey Pietersz, Ian McKenzie, Christina Cheers

(2002), Vol. 20, pp. 1068-1078, Vaccine, Guildford, England, C1-1

journal

Grants

Australian Competitive Grants

Identification of determinants associated with pathogenicity of Swine-Origin 2009 (H1N1) influenza virus

A/Prof John Stambas, A/Prof Stephen Turner, Dr Deborah Middleton

NHMRC - H1N1 Influenza 09

  • 2011: $30,809
  • 2010: $68,985

Evaluation of immune correlates for virus-specific CD8+ T cells following prime-boost vaccination

A/Prof John Stambas

NHMRC Project Grant

  • 2011: $2,064
  • 2010: $159,691

Development of recombinant influenza-SIV vaccines

A/Prof John Stambas, Dr Robert De Rose

University of Melbourne - Aust Centre for HIV and Hepatitis Virology Research

  • 2009: $66,000

Comparative effectiveness of vaccine-induced SIV-specific CD8 T cells

Prof Steven Kent, A/Prof John Stambas

NHMRC Project Grant

  • 2012: $90,000
  • 2011: $90,000

Understanding immunity in an encephalitic mouse model of Hendra virus infection

A/Prof John Stambas, Prof Paul Hertzog, Prof Carola Vinuesa, Dr Deborah Middleton, Dr Linfa Wang

NHMRC Hendra Virus Urgent Call for Research

  • 2014: $90,600
  • 2013: $28,305
  • 2012: $187,450

Impact of Influenza A infection on T cell-mediated immunity to pulmonary tuberculosis

Prof Warwick Britton, Dr Jamie Triccas, Dr Manuela Florido, A/Prof John Stambas

NHMRC Project Grant

  • 2013: $25,000

Development of apocynin as a new antiviral for emerging infectious diseases

A/Prof John Stambas

CSIRO Flagship Collaboration Research Fund - Commonwealth Scientific and Industrial Research Organisation

  • 2013: $85,000

Other Public Sector Funding

The role of endothelium in the pathogenicity of H5N1 HPAI in chickens

A/Prof John Stambas, Ms Jasmina Luczo

  • 2014: $14,000
  • 2013: $28,000
  • 2012: $42,000
  • 2011: $14,000

Design and development of superior pan-diagnostics for Foot-and-Mouth Disease (FMD)

A/Prof John Stambas, Miss Alyssa Van Dreumel

  • 2015: $2,136
  • 2014: $4,272
  • 2013: $4,272
  • 2012: $4,272

Delivery of MIRNA Using Genetically Modified Influenza Virus

A/Prof John Stambas, Prof Alister Ward, Prof Ken Walder, Ms Yingju Xia

  • 2014: $60,000

Other Funding Sources

Collaborative High Bio-Containment immunological Research Facility

Prof Alister Ward, Dr Linfa Wang, A/Prof John Stambas, Dr John Lowenthal, Prof Ralph Tripp, Prof Johnson Mak, Prof Bill Buttemer, Prof Tania De Koning-Ward

  • 2012: $105,000
  • 2011: $100,000

Supervisions

Principal Supervisor
2017

Meagan McMahon

Thesis entitled: The role of ADAMTS enzymes in influenza virus immunity

Doctor of Philosophy (Medicine), School of Medicine

2016

Alyssa Van Dreumel

Thesis entitled: Design and development of Pan-serodiagnostics for Foot-and-mouth disease

Doctor of Philosophy (Medicine), School of Medicine

Jasmina Luczo

Thesis entitled: Pathogenesis of avian influenza: role of the haemagglutinin cleavage site motif

Doctor of Philosophy (Medicine), School of Medicine

2015

Mohammed Jasim Mohammed Shallal

Thesis entitled: Recombinant Influenza virus as HIV vaccine vector

Doctor of Philosophy (Medicine), School of Medicine

Associate Supervisor
2016

Corinna Pade

Thesis entitled: Dissecting the early entry steps of HIV-1 infection

Doctor of Philosophy (Medicine), School of Medicine

2015

Brian Clarke

Thesis entitled: Expression of anti-viral shRNA molecules in transgenic zebrafish

Doctor of Philosophy (Medicine), School of Medicine

2012

Katherine Birrer

Thesis entitled: Silencing Host Genes Using RNAi to Impact Virus Growth in vitro Methods for reducing cotton wax.

MAppSc (Medicine) (High cost), School of Medicine