Prof Johnson Mak

STAFF PROFILE

Position

Chair In Infectious Diseases

Faculty

Faculty of Health

Department

School of Medicine

Campus

CSIRO

Qualifications

Bachelor of Science, McGill University, 1991
Doctor of Philosophy, McGill University, 1996

Biography summary

Education

1991  McGill University, Canada.  B.Sc.  Biochemistry
1996  McGill University, Canada.  Ph.D.  Experimental Medicine (Virology)

Professional Experience
1991 - 1996 Ph.D. training, McGill AIDS Centre, Montreal, Canada.
1996 - 2010 Research Officer, Senior Research Officer, Senior Research Fellow, and Principle Research Fellow, The Macfarlane Burnet Institute for Medical Research, Melbourne, Australia.
1999 - 2010 Lecturer, Senior Lecturer, Associate Professor, Department of Biochemistry and Molecular Biology; Department of Microbiology, Faculty of Medicine, Monash University, Clayton, Australia.
2003 - 2006 Inaugural Chair, Burnet Virology Program, The Macfarlane Burnet Institute for Medical Research and Public Health
2010 - 2014 Australian Research Council Future Fellow, Deakin University; CSIRO AAHL
2011 - Current Chair, Infectious Diseases, School of Medicine, Faculty of Health, Deakin University
2011 - Current Head, HIV and Emerging Virus Laboratory, CSIRO AAHL

Honours and Awards
1993 - 1996 Canadian National Health Research and Development Program (NHRDP) Ph.D. Training Fellowship.
1996 Canadian NHRDP Post-Doctoral Fellowship, awarded to train in a Howard Hughes laboratory. The fellowship was declined by the applicant.
1996 - 1997 Macfarlane Burnet Centre Fellowship.
1998 - 2001 Australian NHMRC Peter Doherty Fellowship
2002 - 2009 Monash Logan Research Fellowship
2002 Otto Basler Travel Grant
2004 - 2008 Pfizer Foundation Fellowship
2005 CASS Foundation Travel Award
2006 CASS Foundation Travel Award
2010 - 2014 Australian Research Council Future Fellow
2011  Frank Fenner Award, Australian Society for Microbiology
2011  Jane Allen Lecture, Cambridge Infectious Diseases, Cambridge University
2015  Outstanding Australian NHMRC External Assessment (Top 0.5% in the country)

Professional Discipline and Peer Review

Member: American Society for Microbiology (ASM-USA), Australian Society for HIV Medicine (ASHM), Australian Society for Medical Research (ASMR), Australian Society of Biochemistry and Molecular Biology (ASBMB-Aus), Australian Society of Microbiology (ASM-Aus), American Society of Biochemistry and Molecular Biology (ASBMB-USA).

2006 - 2012 Executive of Victorian Infection and Immunity Network (formerly known as Monash Infection and Immunity Network)
2008 - 2012 Vice President of Corporate Affairs of Australian Society for Microbiology
2010  Member, Organizing committee for the 2011 Inaugural Lorne Infection and Immunity Meeting

1999 - Current NHMRC and ARC External Grant Assessors

2002 - 2012 NHMRC Grant Reveiw Panel Member (4x), Deputy Chair (1x), Chair (2x).

Journal Editorial: Retrovirology (Editorial Broad, Associate Editor, 2004-Current), Virology Journal (Section Editor, 2004-Current),Viruses (Guest Editor, 2015).

Career highlights

1993 - 1996 Canadian National Health Research and Development Program (NHRDP) Ph.D. Training Fellowship.

1998 - 2001 Australian NHMRC Peter Doherty Fellowship

2002 - 2009 Monash Logan Fellow, Department of Biochemistry and Molecular Biology, Monash University

2004 - 2009 Pfizer Foundation Fellow, The Macfarlane Burnet Institute for Medical Research and Public Health; Department of Biochemistry and Molecular Biology, Monash University

2010 - 2014 Australian Research Council Future Fellow, Deakin University; CSIRO AAHL

2011  Frank Fenner Award, Australian Society for Microbiology

2011  Jane Allen Lecture, Cambridge Infectious Diseases, Cambridge University

Research interests

HIV, viruses, emerging infectious diseases, molecular biology, cell biology, protein biochemistry, protein biophysics, next generation sequencing, fluorescent imaging, super-resolution microscopy, cryo-electron microscopy, virus entry and assembly, virus-host cell interplay, antiviral discovery, host-restriction factor discovery, structural biology

Affiliations

Visiting Scientist, CSIRO AAHL

Teaching interests

Viruses, Microbiology, Medical Innovation, Biotechnology

Units taught

HMM 302

http://www.deakin.edu.au/future-students/courses/unit.php?unit=HMM302&return_to=%2Ffuture-students%2Fcourses%2Fcourse.php%3Fcourse%3DS323%26stutype%3Dlocal

HMM 303

http://www.deakin.edu.au/future-students/courses/unit.php?unit=HMM303&return_to=%2Ffuture-students%2Fcourses%2Fdetail.php%3Fcustomer_cd%3DC%26service_item%3DS323%26version_number%3D3%26element_cd%3DMAJORS-STRUCTURE%26sub_item_number%3D7%26return_to%3D%252Ffuture-students%252Fcourses%252Fcourse.php%253Fcourse%253DS323

HMH 401

http://www.deakin.edu.au/future-students/courses/unit.php?unit=HMH401&return_to=%2Ffuture-students%2Fcourses%2Fcourse.php%3Fcourse%3DH413

Knowledge areas

HIV, viruses, emerging infectious diseases, molecular biology, cell biology, protein biochemistry, protein biophysics, next generation sequencing, fluorescent imaging, super-resolution microscopy, cryo-electron microscopy, virus entry and assembly, virus-host cell interplay, antiviral discovery, host-restriction factor discovery, structural biology

Professional activities

Patents
1998 Provisional patent for the design of a new generation of gene delivery vector for clinical trial.
1999  Full International Patent - “Novel Retrovirus Constructs”
  US Application No. 60/079,772.
  International application No. PCT/AU99/00219.
2003  Provisional patent for the compositions and methods for the prevention and treatment of pathogenic infections.

Professional Discipline
2000 -  Member, American Society for Microbiology (ASM-USA).
2001 -  Member, Australian Society for HIV Medicine (ASHM).
2001 -  Member, Australian Society for Medical Research (ASMR).
2001 - Member of Victorian branch ASMR organizing committee for Medical Research Week.
2002 - Member, Australian Society of Biochemistry and Molecular Biology (ASBMB-Aus).
2002 -  Member, Australian Society of Microbiology (ASM-Aus).
2004 -   Member, National Scientific Advisory Council for ASM-Aus.
2005 - 2009 Member, Organizing Committee for 2005 to 2009 ASHM
2005 - 2009 Member, Organizing Committee for 2005, 08, 09 ACH2
2006   Chair, Division of Virology, Organizing Committee for 2006 ASM-Aus General Meeting
2006 - 2012 Executive of Victorian Infection and Immunity Network (formerly known as Monash Infection and Immunity Network)
2008 - 2012 Vice President of Corporate Affairs of Australian Society for Microbiology
2010  Member, Organizing committee for the 2011 Inaugural Lorne Infection and Immunity Meeting
2010 -  Member, American Society of Biochemistry and Molecular Biology (ASBMB-USA)

Session Chair for Scientific Conferences
2001   ASHM
2002   ASHM
2004   ASHM, ACH2
2005   ASHM, ACH2, ASM
2006  ASHM, ACH2, ASM, Lorne Protein Conference, Australian-China Biomedical Research Conference
2007  CSHL Retroviruses, IAS Meeting
2008 - 10 ASM and ACH2 conferences
2010  CSHL Retroviruses, Lorne Infection and Immunity
2011 Lorne Infection and Immunity
2014 - 15  CSHL Retroviruses


Peer review
1999 -   NHMRC grant assessor.
2001  ICAAP (International Congress on AIDS in Asia and Pacific) reviewer
2002 - Ad hoc reviewer for J Biol Chem, PLoS Med, J Virol, EMBO J, FEBS Lett, Trends in Micro, Chem & Biol, J Leuko Biol, J Mol Biol, Retrovirology, Virology, Arch Virol, Future Virol,, J Gen Virol, Immuno Cell Biol, J Med Virol, JVirol Methods, J Viral Entry, Viruses and J BioMed Sci.
2002   Basic science rapporteur for 2002 ASHM
2003 - 2008 Member of NHMRC Grant Review Panel, Microbiology; Deputy Chair 2006 and Chair for 2007 & 2008.
2004   Basic science rapporteur for 2004 ASHM
2004 -  Member of Editorial Boards: Retrovirology, Virology Journal.
2007 -  Grant assessor for MRC UK, Wellcome Trust, the Netherlands AIDS Fond, The Netherlands Organization for Scientific Research, Prostate Cancer Foundation Australia, and Research Grants Council (Hong Kong).
2011 - Member of Editorial Boards: Future Virology, World Journal of Biological Chemistry, World Journal of Pharmacology
2011 - 2013  Panel Member, Australian Eureka Prize for Infectious Diseases
2012 - Member of Editorial Boards: Virus Research, Clinical and Translational Immunology
2012 - Section Editor: Virology Journal
2012  Member of NHMRC Grant Review Panel, Microbiology and Virology
2013 -  Associate Editor: Retrovirology
2015  Guest Editor for Viruses: Next Generation Sequencing Special Issue

Projects

Project 1: Dissecting the early steps of HIV infection

Viruses are considered neither live nor dead, and are therefore thought to be inert until after entering the host cells. Using cryo-electron microscopy (cryo-EM) and single molecule fluorescence imaging, we have reported that HIV undergo a previously unknown pre-entry priming size expansion event upon receptor engagement, challenging the dogma of viral entry, more specifically, we showed that cell-free viruses are able to perform biological process as seen with live organism. These virus expansion events are induced by engagement of the receptor-binding site of the viral envelope (Env), and involves the re-modelling of multiple virus compartments. Using Mass-spectrometry analysis, we have identified a number of candidate cellular proteins that are involved in this process. The objective of this project is to use in silico approach and siRNA knock down system to evaluate their contribution in this process. This project utilizes a combination of virology, molecular biology, tissue culture and cell biology approaches.

Project 2: Understanding the formation of HIV particles

HIV protein Gag drives the formation of virus particle. During HIV assembly, roughly 2500-5000 Gag molecules will come together for the assembly of immature particle, which is followed by a proteolytic processing maturation to generate infectious virus particles. The formation of virus particles and maturation process is highly regulated, and the smallest interference of these events can block the replication of HIV. The objective of this proposal is to use a combination of protein biochemistry and biophysics to define the mechanism of HIV assembly for the development of novel anti-viral. This project utilizes a combination of molecular biology, protein biochemistry, protein biophysics and electron microscopy approaches.

Project 3: Finding the HIV latently infected cells

With combinational anti-retroviral therapy (cART), HIV infected individuals who have access to these treatments can live a relatively normal life with life expectancy approaching non-infected individuals. However, the last trace of HIV latently infected cells in the body has kept these HIV infected individual to maintain their drug intake for the rest of their lives. Our inability to target, to quantify or to locate these latently infected cells represents the only obstacle to cure HIV. It has recently appreciated that many of these latently infected cells might have produce between 1-1000 copies of viral RNA despite no HIV protein is produced. The objective of this proposal is to provide proof of concept data to fluorescently tag HIV infected cells post-infection. Our design will light up HIV infected cells even these cells have entered viral latency, allowing us to have a means to identify the sanctuary sites of HIV latently infected cells in vivo. This project utilizes a combination of virology, molecular biology and tissue culture approaches.

Publications

Filter by

2016

Estimating the in-vivo HIV template switching and recombination rate

Dr Deborah Cromer, Andrew J. Grimm, Timothy E. Schlub, Prof Johnson Mak, A/Prof Miles Davenport

(2016), Vol. 30, pp. 185-192, AIDS : official journal of the international AIDS society, Philadelphia, Pa., C1

journal
2015

A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library.

Ms Caryll Waugh, Dr Deborah Cromer, Andrew J. Grimm, Abha Chopra, Simon Mallal, A/Prof Miles Davenport, Prof Johnson Mak

(2015), Vol. 12, pp. 55-, Virology Journal, London, Eng., C1

journal

Mutational interference mapping experiment (MIME) for studying RNA structure and function

Redmond P. Smyth, Laurence Despons, Gong Huili, Serena Bernacchi, Marcel Hijnen, Prof Johnson Mak, Fabrice Jossinet, Li Weixi, Jean-Christophe Paillart, Max von Kleist

(2015), Vol. 12, pp. 866-872, Nature methods, New York, NY, C1

journal

Properties of HIV-1 associated cholesterol in addition to raft formation are important for virus infection

David J Hawkes, Kate Jones, Dr Redmond Smyth, Candida F. Pereira, Robert Bittman, Anthony Jaworowski, Prof Johnson Mak

(2015), Vol. 210, pp. 18-21, Virus research, Amsterdam, The Netherlands, C1

journal

Blocking HIV-1 transmission in the female reproductive tract: from microbicide development to exploring local antiviral responses

Dr Sahar Eid, Niamh Mangan, Paul J. Hertzog, Prof Johnson Mak

(2015), Vol. 4, pp. 1-9, Clinical and translational immunology, London, Eng., C1

journal

Cryo-electron microscopy and single molecule fluorescent microscopy detect CD4 receptor induced HIV size expansion prior to cell entry

Dr Sonny Pham, Thibault Tabarin, Dr Megan Garvey, Miss Corinna Pade, Jeremie Rossy, Paul Monaghan, Dr Alex Hyatt, Till Bocking, Dr Andrew Leis, A/Prof Katharina Gaus, Prof Johnson Mak

(2015), Vol. 486, pp. 121-133, Virology, Amsterdam, The Netherlands, C1

journal
2014

Intracellular dynamics of HIV infection

Dr Janka Petravic, Paula C. Ellenberg, Ming-Liang Chan, Geza Paukovics, Redmond P. Smyth, Prof Johnson Mak, A/Prof Miles Davenport

(2014), Vol. 88, pp. 1113-1124, Journal of Virology, Washington DC, C1

journal

Specific recognition of the HIV-1 genomic RNA by the Gag precursor

Ekram Abd El-Wahab, Redmond P. Smyth, Elodie Mailler, Serena Bernacchi, Valerie Vivet-Boudou, Marcel Hijnen, Fabrice Jossinet, Prof Johnson Mak, Jean-Christophe Paillart, Prof Roland Marquet

(2014), Vol. 5, pp. 1-13, Nature communications, London, England, C1

journal

Expression and purification of soluble recombinant full length HIV-1 Pr55(Gag) protein in Escherichia coli

Dr William McKinstry, Marcel Hijnen, Mr Hanumant Tanwar, Lindsay Sparrow, Dr Sureshbabu Nagarajan, Dr Sonny Pham, Prof Johnson Mak

(2014), Vol. 100, pp. 10-18, Protein expression and purification, Amsterdam, The Netherlands, C1

journal

Fifteen to twenty percent of HIV substitution mutations are associated with recombination

Timothy E. Schlub, Andrew J. Grimm, Redmond P. Smyth, Dr Deborah Cromer, Abha Chopra, Simon Mallal, Dr Vanessa Venturi, Ms Caryll Waugh, Prof Johnson Mak, A/Prof Miles Davenport

(2014), Vol. 88, pp. 3837-3849, Journal of virology, Washington, D.C., C1

journal
2013

Allosteric Modulation of the HIV-1 gp120-gp41 Association Site by Adjacent gp120 Variable Region 1 (V1) N-Glycans Linked to Neutralization Sensitivity

Heidi Drummer, Melissa K. Hill, Anne Maerz, Stephanie Wood, Paul Ramsland, Prof Johnson Mak, Pantelis Poumbourios

(2013), Vol. 9, pp. 1-18, PLOS Pathogens, San Francisco, CA, C1

journal

Improved quantification of HIV-1 infected CD4 + T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection

Hongbing Yang, Elizabeth Yorke, Gemma Hancock, Genevieve Clutton, Nellia Sande, Bryan Angus, Redmond P. Smyth, Prof Johnson Mak, Lucy Dorrell

(2013), Vol. 391, pp. 174-178, Journal of Immunological Methods, Amsterdam, Netherlands, C1

journal

HIV-1 Infection of T cells and macrophages are differentially modulated by virion-associated Hck: a nef-dependent phenomenon

Alyssa Cornall, Prof Johnson Mak, Alison Greenway, Gilda Tachedjian

(2013), Vol. 5, pp. 2235-2252, Viruses, Basel, Switzerland, C1

journal

Visualising single molecules of HIV-1 and miRNA nucleic acids

Kate Jones, Dr Adam Karpala, Bevan Hirst, Kristie Jenkins, Mark L. Tizard, Candida F. Pereira, Dr Andrew Leis, Dr Paul Monaghan, Dr Alex Hyatt, Prof Johnson Mak

(2013), Vol. 14, pp. 1-10, BMC cell biology, London, England, C1

journal

Identifying recombination hotspots in the HIV-1 genome

Redmond P. Smyth, Timothy E. Schlub, Andrew J. Grimm, Ms Caryll Waugh, Paula C. Ellenberg, Amit Chopra, S. Mallal, Dr Deborah Cromer, Prof Johnson Mak, A/Prof Miles Davenport

(2013), pp. -, Journal of Virology, Washington, DC, C1

journal
2012

The origin of genetic diversity in HIV-1

Redmond P. Smyth, A/Prof Miles Davenport, Prof Johnson Mak

(2012), Vol. 169, pp. 415-429, Virus research, Amsterdam, Netherlands, C1

journal

HIV taken by STORM : super-resolution fluorescence microscopy of a viral infection

Candida F. Pereira, Jeremie Rossy, Dylan M. Owen, Prof Johnson Mak, Katherina Gaus

(2012), Vol. 9, pp. 1-6, Virology journal, London, England, C1

journal
2011

HIV infection of dendritic cells subverts the IFN induction pathway via IRF-1 and inhibits type 1 IFN production

Anthony D. Harman, Joey Lai, Dr Stuart Turville, Shamith Samarajiwa, Lachlan Gray, Valerie Marsden, Sarah Mercier, Kate Jones, Najla Nasr, Helen Cumming, Heather Donaghy, Prof Johnson Mak, Melissa Churchill, Paul J. Hertzog, Anthony L. Cunningham

(2011), Vol. 118, pp. 298-308, Blood, Washington, D. C., C1-1

journal

Early events of HIV-1 infection : can signalling be the next therapeutic target?

Kate Jones, Redmond P. Smyth, Candida F. Pereira, P.U Cameron, Sharon R. Lewin, Anthony Jaworowski, Prof Johnson Mak

(2011), Vol. 6, pp. 269-283, Journal of neuroimmune pharmacology, New York, N. Y., C1-1

journal

8-modified-2'-deoxyadenosine analogues induce delayed polymerization arrest during HIV-1 reverse transcription

Valerie Vivet-Boudou, Catherine Isel, Marwan Sleiman, Redmond P. Smyth, Nouha Ben-Gaied, Patrick Barhoum, Geraldine Laumond, Guillaume Bec, Matthias Gotte, Prof Johnson Mak, Anne-Marie Aubertin, Alain Burger, Prof Roland Marquet

(2011), Vol. 6, pp. 1-12, PLoS one, San Francisco, Calif., C1-1

journal

Labeling of multiple HIV-1 proteins with the biarsenical-tetracysteine system

Candida F. Pereira, Paula C. Ellenberg, Kate Jones, Tara L. Fernandez, Redmond P. Smyth, David J Hawkes, Marcel Hijnen, Valerie Vivet-Boudou, Prof Roland Marquet, Iain Johnson, Prof Johnson Mak

(2011), Vol. 6, pp. 1-10, PLoS one, San Francisco, Calif., C1-1

journal
2010

Bacterial membrane vesicles deliver peptidoglycan to NOD1 in epithelial cells

Maria Kaparakis, Lynne Turnbull, Leticia Carneiro, Stephen Firth, Harold A. Coleman, Helena C. Parkington, Lionel Le Bourhis, Abdulgader Karrar, Jerome Viala, Prof Johnson Mak, Melanie L. Hutton, John K. Davies, Peter J. Crack, Paul J. Hertzog, Dana Philpott, Stephen E. Girardin, Cynthia B. Whitchurch, Richard L. Ferrero

(2010), Vol. 12, pp. 372-385, Cellular microbiology, Oxford, England, C1-1

journal

Reducing chimera formation during PCR amplification to ensure accurate genotyping

Redmond P. Smyth, Timothy E. Schlub, Andrew J. Grimm, Dr Vanessa Venturi, A. Chopra, S. Mallal, A/Prof Miles Davenport, Prof Johnson Mak

(2010), Vol. 469, pp. 45-51, Gene, Amsterdam, The Netherlands, C1-1

journal

X4 and R5 HIV-1 have distinct post-entry requirements for uracil DNA glycosylase during infection of primary cells

Kate Jones, Michael Roche, Michael P. Gantier, Nasim A. Begum, Tasuku Honjo, Salvatore Caradonna, Bryan R. G. Williams, Prof Johnson Mak

(2010), Vol. 285, pp. 18603-18614, Journal of biological chemistry, Bethesda, Md., C1-1

journal

Establishment of HIV-1 latency in resting CD4+ T cells depends on chemokine-induced changes in the actin cytoskeleton

P.U Cameron, Suha Saleh, Georgina Sallmann, Ajantha Solomon, Fiona Wightman, Vanessa A. Evans, Genevieve Boucher, Elias K. Haddad, Rafick-Pierre Sekaly, Andrew N. Harman, Jenny L. Anderson, Kate Jones, Prof Johnson Mak, Anthony L. Cunningham, Anthony Jaworowski, Sharon R. Lewin

(2010), Vol. 107, pp. 16934-16939, National academy of science. Proceedings, Washington, D. C., C1-1

journal

Accurately measuring recombination between closely related HIV-1 genomes

Timothy E. Schlub, Redmond P. Smyth, Andrew J. Grimm, Prof Johnson Mak, A/Prof Miles Davenport

(2010), Vol. 6, pp. 1-11, PLoS computational biology, San Francisco, Calif., C1-1

journal
2009

The A-rich RNA sequences of HIV-1 pol are important for the synthesis of viral cDNA

Cameron P. Keating, Melissa K. Hill, David J Hawkes, Redmond P. Smyth, Catherine Isel, Shu-Yun Le, Ann C. Palmenberg, John A. Marshall, Prof Roland Marquet, Gary J. Nabel, Prof Johnson Mak

(2009), Vol. 37, pp. 945-956, Nucleic acids research, Oxford, England, C1-1

journal

An antiviral response directed by PKR phosphorylation of the RNA helicase A

Anthony J. Sadler, Olivier Latchoumanin, David J Hawkes, Prof Johnson Mak, Bryan R. G. Williams

(2009), Vol. 5, pp. 1-11, PLoS pathogens, San Francisco, Calif., C1-1

journal
2008

Primary T-lymphocytes rescue the replication of HIV-1 DIS RNA mutants in part by facilitating reverse transcription

Kate Jones, Secondo Sonza, Prof Johnson Mak

(2008), Vol. 36, pp. 1578-1588, Nucleic acids research, Oxford, England, C1-1

journal
2007

Alteration of the proline at position 7 of the HIV-1 spacer peptide p1 suppresses viral infectivity in a strain dependent manner

Melissa K. Hill, Anna Bellamy-McIntyre, Laura J. Vella, Shahan M. Campbell, John A. Marshall, Gilda Tachedjian, Prof Johnson Mak

(2007), Vol. 5, pp. 69-78, Current HIV research, Bussum, Netherlands, C1-1

journal
2006

Lipid membrane : a novel target for viral and bacterial pathogens

David J Hawkes, Prof Johnson Mak

(2006), Vol. 7, pp. 1615-1621, Current drug target, Bussum, Netherlands, C1-1

journal

Potent nonnucleoside reverse transcriptase inhibitors target HIV-1 gag-pol

Anna Figueiredo, Katie L. Moore, Prof Johnson Mak, Nicolas Sluis-Cremer, Marie-Pierre de Bethune, Gilda Tachedjian

(2006), Vol. 2, pp. 1051-1059, PLoS pathogens, San Francisco, Calif., C1-1

journal
2005

The packaging and maturation of the HIV-1 Pol proteins

Melissa K. Hill, Gilda Tachedjian, Prof Johnson Mak

(2005), Vol. 3, pp. 73-85, Current HIV research, Bussum, Netherlands, C1-1

journal

Innate immunity and intracellular trafficking : insights for novel anti-HIV-1 therapeutics

Kate Jones, Prof Johnson Mak

(2005), Vol. 3, pp. 97-117, Current pharmacogenomics and personalized medicine, Bussum, Netherlands, C1-1

journal

Analysis of the contribution of reverse transcriptase and integrase proteins to retroviral RNA dimer conformation

Penelope Buxton, Gilda Tachedjian, Prof Johnson Mak

(2005), Vol. 79, pp. 6338-6348, Journal of virology, Washington, D. C., C1-1

journal

Mutations that abrogate human immunodeficiency virus type 1 reverse transcriptase dimerization affect maturation of the reverse transcriptase heterodimer

Johanna Wapling, Katie L. Moore, Secondo Sonza, Prof Johnson Mak, Gilda Tachedjian

(2005), Vol. 79, pp. 10247-10257, Journal of virology, Washington, D. C., C1-1

journal
2004

The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity

Shahan M. Campbell, Katherina Gaus, Robert Bittman, Wendy Jessup, Suzanne M. Crowe, Prof Johnson Mak

(2004), Vol. 78, pp. 10556-10565, Journal of virology, Washington, D. C., C1-1

journal

Nef binds p6* in gagpol during replication of human immunodeficiency virus type 1

Luciana J. Costa, Yong-Hui Zheng, Jerica Sabotic, Prof Johnson Mak, Oliver T. Fackler, B. Matija Peterlin

(2004), Vol. 78, pp. 5311-5323, Journal of virology, Washington, D. C., C1-1

journal

Dimerization of retroviral RNA genomes : an inseparable pair

Jean-Christophe Paillart, M. Shehu-Xhilaga, Prof Roland Marquet, Prof Johnson Mak

(2004), Vol. 2, pp. 461-472, Nature reviews. Microbiology, London, England, C1-1

journal
2003

Defective phagocytosis by human monocyte/macrophages following HIV-1 infection : underlying mechanisms and modulation by adjunctive cytokine therapy

Katherine Kedzierska, Rula Azzam, Philip Ellery, Prof Johnson Mak, Anthony Jaworowski, Suzanne M. Crowe

(2003), Vol. 26, pp. 247-263, Journal of clinical virology, Amsterdam, The Netherlands, C1-1

journal

The dimer initiation sequence stem-loop of human immunodeficiency virus type 1 is dispensable for viral replication in peripheral blood mononuclear cells

Melissa K. Hill, M. Shehu-Xhilaga, Shahan M. Campbell, P. Poumbourios, Suzanne M. Crowe, Prof Johnson Mak

(2003), Vol. 77, pp. 8329-8335, Journal of virology, Washington, D. C., C1-1

journal

Human immunodeficiency virus type 1 protease regulation of tat activity is essential for efficient reverse transcription and replication

Ann Apolloni, C. W. Hooker, Prof Johnson Mak, D. Harrich

(2003), Vol. 77, pp. 9912-9921, Journal of virology, Washington, D. C., C1-1

journal
2002

Virion-associated cholesterol is critical for the maintenance of HIV-1 structure and infectivity

Shahan M. Campbell, Suzanne M. Crowe, Prof Johnson Mak

(2002), Vol. 16, pp. 2253-2261, AIDS, Philadelphia, Pa., C1-1

journal

HIV-1 down-modulates γ signaling chain of FcγR in human macrophages : a possible mechanism for inhibition of phagocytosis

Katherine Kedzierska, Philip Ellery, Prof Johnson Mak, Sharon R. Lewin, Suzanne M. Crowe, Anthony Jaworowski

(2002), Vol. 168, pp. 2895-2903, Journal of immunology, Bethesda, Md., C1-1

journal

Overexpression and incorporation of GagPol precursor does not impede packaging of HIV-1 tRNALys3 but promotes intracellular budding of virus-like particles

M. Shehu-Xhilaga, J.-Y. Lee, Shahan M. Campbell, John A. Marshall, Suzanne M. Crowe, Prof Johnson Mak

(2002), Vol. 9, pp. 697-705, Journal of biomedical science, Amsterdam, The Netherlands, C1-1

journal

Proline residues within spacer peptide p1 are important for human immunodeficiency virus type 1 infectivity, protein processing, and genomic RNA dimer stability

Melissa K. Hill, M. Shehu-Xhilaga, Suzanne M. Crowe, Prof Johnson Mak

(2002), Vol. 76, pp. 11245-11253, Journal of virology, Washington, D. C., C1-1

journal

The conformation of the mature dimeric human immunodeficiency virus type 1 RNA genome requires packaging of pol protein

M. Shehu-Xhilaga, Melissa K. Hill, John A. Marshall, J. Kappes, Suzanne M. Crowe, Prof Johnson Mak

(2002), Vol. 76, pp. 4331-4340, Journal of virology, Washington, D. C., C1-1

journal
2001

nef-deleted HIV-1 inhibits phagocytosis by monocyte-derived macrophages in vitro but not by peripheral blood monocytes in vivo

Katherine Kedzierska, Prof Johnson Mak, Anthony Jaworowski, Alison Greenway, Antoniette Violo, HiuTat Chan, Dr Jane Hocking, Damian F.J. Purcell, John S. Sullivan, John Mills, Suzanne M. Crowe

(2001), Vol. 15, pp. 945-955, AIDS, Philadelphia, Pa., C1-1

journal

Maintenance of the gag/gag-pol ratio is important for human immunodeficiency virus type 1 RNA dimerization and viral infectivity

M. Shehu-Xhilaga, Suzanne M. Crowe, Prof Johnson Mak

(2001), Vol. 75, pp. 1834-1841, Journal of virology, Washington, D.C., C1-1

journal

Lipid rafts and HIV-1 : from viral entry to assembly of progeny virions

Shahan M. Campbell, Suzanne M. Crowe, Prof Johnson Mak

(2001), Vol. 22, pp. 217-227, Journal of clinical virology, Amsterdam, The Netherlands, C1-1

journal

Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1

Melissa K. Hill, C. W. Hooker, D. Harrich, Suzanne M. Crowe, Prof Johnson Mak

(2001), Vol. 75, pp. 6835-6840, Journal of virology, Washington, D. C., C1-1

journal

Proteolytic processing of the P2/nucleocapsid cleavage site is critical for human immunodeficiency virus type 1 RNA dimer maturation

M. Shehu-Xhilaga, H. G. Kraeusslich, S. Pettit, R. Swanstrom, J.-Y. Lee, John A. Marshall, Suzanne M. Crowe, Prof Johnson Mak

(2001), Vol. 75, pp. 9156-9164, Journal of virology, Washington, D. C., C1-1

journal
2000

Granulocyte-macrophage colony-stimulating factor inhibits HIV-1 replication in monocyte-derived macrophages

Katherine Kedzierska, Anne Maerz, Tammra Warby, Anthony Jaworowski, HiuTat Chan, Prof Johnson Mak, Secondo Sonza, Angel Lopez, Suzanne M. Crowe

(2000), Vol. 14, pp. 1739-1748, AIDS, Philadelphia, Pa., C1-1

journal

Granulocyte-macrophage colony-stimulating factor augments phagocytosis of mycobacterium avium complex by human immunodeficiency virus type 1-infected monocytes/macrophages in vitro and in vivo

Katherine Kedzierska, Prof Johnson Mak, Anne Mijch, Mr Ian Cooke, Melissa Rainbird, Sally Roberts, Geza Paukovics, A/Prof Damien Jolley, Angel Lopez, Suzanne M. Crowe

(2000), Vol. 181, pp. 390-394, Journal of infectious diseases, Cary, N. C., C1-1

journal
1999

Effect of insulin-like growth factor I on HIV type 1 long terminal repeat-driven chloramphenicol acetyltransferase expression

Ralph J. Germinario, S. P. Colby-Germinario, A. Acel, R. Chandok, K. Davison, Prof Johnson Mak, L. Kleiman, E. Faust, M. A. Wainberg

(1999), Vol. 15, pp. 829-836, AIDS research and human retroviruses, New Rochelle, N. Y., C1-1

journal
1997

Primer tRNAs for reverse transcription

Prof Johnson Mak, L. Kleiman

(1997), Vol. 71, pp. 8087-8095, Journal of virology, Washington, D. C., C1-1

journal

Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization

Michael Laughrea, Louis Jette, Prof Johnson Mak, L. Kleiman, Chen Liang, M. A. Wainberg

(1997), Vol. 71, pp. 3397-3406, Journal of virology, Washington, D. C., C1-1

journal

Effects of mutations in Pr160gag-pol upon tRNAlys3 and Pr160gag-pol incorporation into HIV-1

Prof Johnson Mak, Ahmad Khorchid, Qun Cao, Yue Huang, Israel Lowy, Michael A. Parniak, Vinayaka R. Prasad, M. A. Wainberg, L. Kleiman

(1997), Vol. 265, pp. 419-431, Journal of molecular biology, London, England, C1-1

journal
1996

Multiple forms of tRNAlys3 in HIV-1

Zhuo Li, Ann Shalom, Yue Huang, Prof Johnson Mak, Eric Arts, M. A. Wainberg, L. Kleiman

(1996), Vol. 227, pp. 530-540, Biochemical and biophysical research communication, Maryland Heights, Mo., C1-1

journal

Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription

Yue Huang, Ann Shalom, Zhuo Li, Dr Jing Wang, Prof Johnson Mak, M. A. Wainberg, L. Kleiman

(1996), Vol. 70, pp. 4700-4706, Journal of virology, Washington, D. C., C1-1

journal
1994

Incorporation of excess wild-type and mutant tRNA(3Lys) into human immunodeficiency virus type 1

Yue Huang, Prof Johnson Mak, Qun Cao, Zhuo Li, M. A. Wainberg, L. Kleiman

(1994), Vol. 68, pp. 7676-7683, Journal of virology, Washington, D. C., C1-1

journal

Mature reverse transcriptase (p66/p51) is responsible for low levels of viral DNA found in human immunodeficiency virus type 1 (HIV-1)

Eric Arts, Prof Johnson Mak, L. Kleiman, M. A. Wainberg

(1994), Vol. 8, pp. S175-S178, Leukemia, London, England, C1-1

journal

Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles

Prof Johnson Mak, Min Jiang, M. A. Wainberg, Marie-Louise Hammarskjold, David Rekosh, L. Kleiman

(1994), Vol. 68, pp. 2065-2072, Journal of virology, Washington, D. C., C1-1

journal

Reverse transcriptase is an important factor for the primer tRNA selection in HIV-1

Min Jiang, Prof Johnson Mak, Yue Huang, Lawrence Kleiman

(1994), Vol. 8, pp. S149-S151, Leukemia, London, England, C1-1

journal

DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity

Eric Arts, Prof Johnson Mak, L. Kleiman, M. A. Wainberg

(1994), Vol. 75, pp. 1605-1613, Journal of general virology, London, England, C1-1

journal

Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication

Xuguang Li, Prof Johnson Mak, Eric Arts, Zhengxian Gu, L. Kleiman, M. A. Wainberg, Michael A. Parniak

(1994), Vol. 68, pp. 6198-6206, Journal of virology, Washington, D. C., C1-1

journal
1993

Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1

Min Jiang, Prof Johnson Mak, Azim Ladha, Eric Cohen, Michel Klein, Benjamin Rovinski, L. Kleiman

(1993), Vol. 67, pp. 3246-3253, Journal of virology, Washington, D. C., C1-1

journal
1992

Variable tRNA content in HIV-1IIIB

Min Jiang, Prof Johnson Mak, M. A. Wainberg, Michael A. Parniak, Eric Cohen, L. Kleiman

(1992), Vol. 185, pp. 1005-1015, Biochemical and biophysical research communication, Maryland Heights, Mo., C1-1

journal