SLE321 - Molecular Biology Techniques
|Year||2016 unit information|
|Enrolment modes:||Trimester 1: Burwood (Melbourne)|
|Previously coded as:|
One of SLE206, SLE221, SLE234 or SLE254
3 x 1 hour class per week, 6 x 3 hour practicals per trimester.
Molecular biology techniques are common methods used in cell and molecular biology which involve manipulation nucleic acid. SLE321 is designed for students to apply knowledge of molecular biology technique in the fields of biochemistry, genetics, and medicine. In this unit, student will learn concepts and latest methodological advances in molecular biology and examine the varied applications of recombinant DNA techniques to forensics, medicine, agriculture and systematics and ecology. In practical sessions, students will learn essential skills in molecular biological experimentation. They will be required to apply knowledge that relates to modern molecular biology techniques to investigate the isolation and transformation of gene from different plasmids. They will also learn principles of gel electrophoresis, extraction and analysis of DNA, polymerase chain reaction (PCR)-mediated amplification and identification of DNA molecules, cloning and expression of DNA fragments or genes to explore the importance of gene expression and regulation.
In-class test 20%, practical report 35%, examination 45%