Dr Greg Kowalski

STAFF PROFILE

Position

Lecturer, Medical Sciences

Faculty

Faculty of Health

Department

School of Medicine

Campus

Geelong Waurn Ponds Campus

Qualifications

Doctor of Philosophy, Monash University, 2012

Contact

greg.kowalski@deakin.edu.au
+61 3 924 46882

Biography

My research spans the metabolic biochemistry, integrative physiology, endocrinology and analytical chemistry fields. Specifically, I am interested in insulin and glucagon secretion and action as well as understanding how pathways of central carbon metabolism (glucose, lipids, amino acids) are coordinated across different organ systems (liver, muscle, pancreas, adipose) in vivo. A particular interest is in the organ specific evolution of insulin resistance and hyperinsulinaemia along with the role they play in disease development, including diabetes and cardiovascular disease. I am also interested in skeletal muscle phospholipid metabolism and how it relates to mitochondrial structure and function. I utilise both human and rodent in vivo experimentation, and am specialised in the use of stable isotope metabolic tracer methodology and quantitative targeted metabolomics. I also manage the biological gas chromatography-mass spectrometry platform within the Metabolic Research Unit at the School of Medicine. 

Read more on Greg's profile

Research interests

Type 2 Diabetes 

Hepatic intermediary metabolism

Insulin Resistance

Energy Metabolism

Gluconeogenesis

Endocrinology

Units taught

Topic Coordinator: HME912 Cardiorespiratory Physiology (Medical Degree)

Guest Lecturer: HME911 Human Biology (Medical Degree)

Awards

2018-2020: Australian Research Council Discovery Early Career Research Award (ARC DECRA) 

2020: Diabetes Australia (DART) Project Grant (CIA Kowalski $60,000)

2019: Diabetes Australia (DART) Project Grant (CIA Kowalski $60,000)

2016: Diabetes Australia (DART) Project Grant (CIA Kowalski $60,000) 

2015: Diabetes Australia (DART) Project Grant (CIA Kowalski $60,000)

202016-2017: Alfred Deakin Postdoctoral Research Fellowship

2013: Australian Diabetes Society (ADS): Pincus Taft Young Investigator Award ($2000)

 
2012: ADS Basic Science Poster Award ($500)

2008 -Recipient of Baker IDI Heart & Diabetes Institute Bright Sparks Scholarship; a three year top-up scholarship ($6000 per year)


2008-Recipient of two post-graduate research scholarships:
-Australian Post-Graduate Award (APA), Monash University
-Melbourne University Research Scholarship (turned down to take up APA)

Publications

Filter by

2021

Autophagy is not involved in lipid accumulation and the development of insulin resistance in skeletal muscle

María Morales-Scholz, Courtney Swinton, Robyn Murphy, Greg Kowalski, Clinton Bruce, Kirsten Howlett, Christopher Shaw

(2021), Vol. 534, pp. 533-539, Biochemical and Biophysical Research Communications, Amsterdam, The Netherlands, C1

journal article

Insulin resistance in type 1 diabetes managed with metformin (INTIMET): study protocol of a double‐blind placebo‐controlled, randomised trial

Jennifer Snaith, Dorit Samocha‐Bonet, Jennifer Evans, Zhixin Liu, Greg Kowalski, Clinton Bruce, Deborah Holmes‐Walker, Jerry Greenfield

(2021), pp. 1-9, Diabetic medicine, Chichester, Eng., C1

journal article

Translating glucose tolerance data from mice to humans: Insights from stable isotope labelled glucose tolerance tests

Clinton Bruce, Steven Hamley, W Ang, Kirsten Howlett, Christopher Shaw, Greg Kowalski

(2021), Vol. 53, pp. 1-11, Molecular metabolism, Amsterdam, The Netherlands, C1

journal article

Baseline serum amino acid levels predict treatment response to augmentation with N-acetylcysteine (NAC) in a bipolar disorder randomised trial

C Bortolasci, A Turner, M Mohebbi, Z Liu, M Ashton, L Gray, W Marx, A Walker, G Kowalski, F Jacka, M Berk, O Dean, K Walder

(2021), Vol. 142, pp. 376-383, Journal of Psychiatric Research, Amsterdam, The Netherlands, C1

journal article
2020

The effects of early-onset pre-eclampsia on placental creatine metabolism in the third trimester

S Ellery, P Murthi, P Gatta, A May, M Davies-Tuck, G Kowalski, D Callahan, C Bruce, E Wallace, D Walker, H Dickinson, R Snow

(2020), Vol. 21, pp. 1-14, International journal of molecular sciences, Basel, Switzerland, C1

journal article

Mapping the Associations of the Plasma Lipidome With Insulin Resistance and Response to an Oral Glucose Tolerance Test

H Beyene, S Hamley, C Giles, K Huynh, A Smith, M Cinel, N Mellet, M Morales-Scholz, D Kloosterman, K Howlett, G Kowalski, C Shaw, D Magliano, C Bruce, P Meikle

(2020), Vol. 105, pp. e1041-e1055, The Journal of clinical endocrinology and metabolism, Oxford, Eng., C1

journal article

Dynamic glucose disposal is driven by reduced endogenous glucose production in response to voluntary wheel running: A stable isotope approach

T Allerton, G Kowalski, H Hang, J Stephens

(2020), Vol. 319, pp. E2-E10, American Journal of Physiology - Endocrinology and Metabolism, Bethesda, Md., C1

journal article

Ultrahigh-resolution mass spectrometry method for resolving 13C-Enrichment patterns in a microalgal lipidome

Sheik Elahee Doomun, Shuai Nie, Stella Loke, Greg Kowalski, Peter Beech, Damien Callahan

(2020), Vol. 31, pp. 1763-1772, Journal of the American Society for Mass Spectrometry, Washington, D.C., C1

journal article

The assimilation of glycerol into lipid acyl chains and associated carbon backbones of Nannochloropsis salina varies under nitrogen replete and deplete conditions

N Poddar, S Elahee Doomun, D Callahan, Greg Kowalski, G Martin

(2020), Biotechnology and Bioengineering, Hoboken, N.J., C1

journal article

An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation, and markers of postnatal myogenesis

J Mikovic, C Brightwell, A Lindsay, Y Wen, G Kowalski, A Russell, C Fry, S Lamon

(2020), Vol. 319, pp. E1008-E1018, American Journal of Physiology - Endocrinology and Metabolism, Bethesda, Md., C1

journal article

Loss of protein kinase D activity demonstrates redundancy in cardiac glucose metabolism and preserves cardiac function in obesity

K De Jong, L Hall, M Renton, T Connor, S Martin, G Kowalski, C Shaw, C Bruce, K Howlett, S McGee

(2020), Vol. 42, pp. 1-11, Molecular metabolism, Amsterdam, The Netherlands, C1

journal article

An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice

Timothy Allerton, Greg Kowalski, James Stampley, Brian Irving, John Lighton, Z Floyd, Jacqueline Stephens

(2020), pp. 1-52, Medicine & Science in Sports & Exercise, Philadelphia, Pa., C1

journal article
2019

Postprandial aminogenic insulin and glucagon secretion can stimulate glucose flux in humans

Teddy Ang, Clinton Bruce, Greg Kowalski

(2019), Vol. 68, pp. 939-946, Diabetes, Arlington, Va., C1

journal article

UNICORN babies: understanding circulating and cerebral creatine levels of the preterm infant. An observational study protocol

Mary Berry, Melissa Schlegel, Greg Kowalski, Clinton Bruce, Damien Callahan, Miranda Davies-Tuck, Hayley Dickinson, Angus Goodson, Angie Slocombe, Rod Snow, David Walker, Stacey Ellery

(2019), Vol. 10, pp. 1-8, Frontiers in physiology, Lausanne, Switzerland, C1

journal article

Modest changes to glycemic regulation are sufficient to maintain glucose flux in healthy humans following overfeeding with a habitual macronutrient composition

Dale Morrison, Greg Kowalski, Clinton Bruce, Glenn Wadley

(2019), Vol. 316, pp. E1061-E1070, American journal of physiology. Endocrinology and metabolism, Bethesda, Md., C1

journal article

Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling

Lewin Small, Amanda Brandon, Benjamin Parker, Vinita Deshpande, Azrah Samsudeen, Greg Kowalski, Jane Reznick, Donna Wilks, Elaine Preston, Clinton Bruce, David James, Nigel Turner, Gregory Cooney

(2019), Vol. 25, pp. 107-118, Molecular metabolism, Amsterdam, The Netherlands, C1

journal article

Phosphatidylserine decarboxylase is critical for the maintenance of skeletal muscle mitochondrial integrity and muscle mass

A Selathurai, G Kowalski, S Mason, D Callahan, V Foletta, P Della Gatta, A Lindsay, S Hamley, G Kaur, A Curtis, M Burch, T Ang, S McGee, C Bruce

(2019), Vol. 27, pp. 33-46, Molecular metabolism, Amsterdam, The Netherlands, C1

journal article

Placental creatine metabolism in cases of placental insufficiency and reduced fetal growth

Stacey Ellery, Padma Murthi, Miranda Davies-Tuck, Paul Della Gatta, Anthony May, Greg Kowalski, Damien Callahan, Clinton Bruce, Nicole Alers, Suzanne Miller, Jan Erwich, Euan Wallace, David Walker, Hayley Dickinson, Rod Snow

(2019), Vol. 25, pp. 495-505, Molecular human reproduction, Oxford, Eng., C1

journal article

Mechanisms of hyperinsulinaemia in apparently healthy non-obese young adults: role of insulin secretion, clearance and action and associations with plasma amino acids

S Hamley, D Kloosterman, T Duthie, C Dalla Man, R Visentin, S Mason, T Ang, A Selathurai, G Kaur, M Morales-Scholz, K Howlett, G Kowalski, C Shaw, C Bruce

(2019), Vol. 62, pp. 2310-2324, Diabetologia, Berlin, Germany, C1

journal article

Treatment of type 2 diabetes with the designer cytokine IC7Fc

M Findeisen, T Allen, D Henstridge, H Kammoun, A Brandon, L Baggio, K Watt, M Pal, L Cron, E Estevez, C Yang, G Kowalski, L O’Reilly, C Egan, E Sun, L Thai, G Krippner, T Adams, R Lee, J Grötzinger, C Garbers, S Risis, M Kraakman, N Mellet, J Sligar, E Kimber, R Young, M Cowley, C Bruce, P Meikle, P Baldock, P Gregorevic, T Biden, G Cooney, D Keating, D Drucker, S Rose-John, M Febbraio

(2019), Vol. 574, pp. 63-68, Nature, London, Eng., C1

journal article
2018

Measurement of postprandial glucose fluxes in response to acute and chronic endurance exercise in healthy humans

D Morrison, G Kowalski, E Grespan, A Mari, C Bruce, G Wadley

(2018), Vol. 314, pp. E503-E511, American journal of physiology: endocrinology and metabolism, Bethesda, Md., C1

journal article

AgRP neurons require carnitine acetyltransferase to regulate metabolic flexibility and peripheral nutrient partitioning

A Reichenbach, R Stark, M Mequinion, R Denis, J Goularte, R Clarke, S Lockie, M Lemus, G Kowalski, C Bruce, C Huang, R Schittenhelm, R Mynatt, B Oldfield, M Watt, S Luquet, Z Andrews

(2018), Vol. 22, pp. 1745-1759, Cell reports, Amsterdam, The Netherlands, C1

journal article

Endogenous glucose production following sequential meals in humans: evidence for more prolonged suppression following ingestion of a second meal

Teddy Ang, Greg Kowalski, Clinton Bruce

(2018), Vol. 315, pp. E904-E911, American journal of physiology: endocrinology and metabolism, Bethesda, Md., C1

journal article

A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism

Nigel Turner, Xin Lim, Hamish Toop, Brenna Osborne, Amanda Brandon, Elysha Taylor, Corrine Fiveash, Hemna Govindaraju, Jonathan Teo, Holly McEwen, Timothy Couttas, Stephen Butler, Abhirup Das, Greg Kowalski, Clinton Bruce, Kyle Hoehn, Thomas Fath, Carsten Schmitz-Peiffer, Gregory Cooney, Magdalene Montgomery, Jonathan Morris, Anthony Don

(2018), Vol. 9, Nature communications, London, Eng., C1

journal article
2017

Lysine post-translational modification of glyceraldehyde-3-phosphate dehydrogenase regulates hepatic and systemic metabolism

S Bond, K Howlett, G Kowalski, S Mason, T Connor, A Cooper, V Streltsov, C Bruce, K Walder, S McGee

(2017), Vol. 31, pp. 2592-2602, FASEB journal, Bethesda, Md., C1

journal article

Creatine biosynthesis and transport by the term human placenta

S Ellery, P Della Gatta, C Bruce, G Kowalski, M Davies-Tuck, J Mockler, P Murthi, D Walker, R Snow, H Dickinson

(2017), Vol. 52, pp. 86-93, Placenta, Amsterdam, The Netherlands, C1

journal article

Does maternal-fetal transfer of creatine occur in pregnant sheep?

S Baharom, R De Matteo, S Ellery, P Della Gatta, C Bruce, G Kowalski, N Hale, H Dickinson, R Harding, D Walker, R Snow

(2017), Vol. 313, pp. E75-E83, American journal of physiology, endocrinology and metabolism, Bethesda, Md., C1

journal article

Resolution of glucose intolerance in long-term high-fat, high-sucrose-fed mice

G Kowalski, M Kraakman, S Mason, A Murphy, C Bruce

(2017), Vol. 233, pp. 269-279, Journal of endocrinology, Bristol, Eng., C1

journal article

The effect of ingested glucose dose on the suppression of endogenous glucose production in humans

G Kowalski, S Moore, S Hamley, A Selathurai, C Bruce

(2017), Vol. 66, pp. 2400-2406, Diabetes, Arlington County, Va., C1

journal article
2016

PKR is not obligatory for high-fat diet-induced obesity and its associated metabolic and inflammatory complications

G Lancaster, H Kammoun, M Kraakman, G Kowalski, C Bruce, M Febbraio

(2016), Vol. 7, pp. 1-10, Nature communications, London, Eng., C1

journal article

Overexpression of striated muscle activator of Rho signaling (STARS) increases C2C12 skeletal muscle cell differentiation

M Wallace, P Della Gatta, B Mir, G Kowalski, J Kloehn, M McConville, A Russell, S Lamon

(2016), Vol. 7, pp. 1-13, Frontiers in physiology, Lausanne, Switzerland, C1

journal article

Reversing diet-induced metabolic dysregulation by diet switching leads to altered hepatic de novo lipogenesis and glycerolipid synthesis

G Kowalski, S Hamley, A Selathurai, J Kloehn, D De Souza, S O’Callaghan, B Nijagal, D Tull, M McConville, C Bruce

(2016), Vol. 6, pp. 1-10, Scientific reports, London, Eng., C1

journal article

Strategies for extending metabolomics studies with stable isotope labelling and fluxomics

A Srivastava, G Kowalski, D Callahan, P Meikle, D Creek

(2016), Vol. 6, pp. 1-13, Metabolites, Basel, Switzerland, C1

journal article

Analysis of mammalian cell proliferation and macromolecule synthesis using deuterated water and gas chromatography-mass spectrometry

V Foletta, M Palmieri, J Kloehn, S Mason, S Previs, M McConville, O Sieber, C Bruce, G Kowalski

(2016), Vol. 6, pp. 1-19, Metabolites, Basel, Switzerland, C1

journal article
2015

Overexpression of sphingosine kinase 1 in liver reduces triglyceride content in mice fed a low but not high-fat diet

Greg Kowalski, Joachim Kloehn, Micah Burch, Ahrathy Selathurai, Steven Hamley, Stéphanie Bayol, Séverine Lamon, Matthew Watt, Robert Lee-Young, Malcolm McConville, C Bruce

(2015), Vol. 1851, pp. 210-219, Biochimica et Biophysica Acta (BBA)- molecular and cell biology of lipids, Amsterdam, Netherlands, C1

journal article

Application of dynamic metabolomics to examine in vivo skeletal muscle glucose metabolism in the chronically high-fat fed mouse

G Kowalski, D De Souza, M Burch, S Hamley, J Kloehn, A Selathurai, D Tull, S O'Callaghan, M McConville, C Bruce

(2015), Vol. 462, pp. 27-32, Biochemical and Biophysical Research Communications, C1

journal article

The CDP-Ethanolamine Pathway Regulates Skeletal Muscle Diacylglycerol Content and Mitochondrial Biogenesis without Altering Insulin Sensitivity.

A Selathurai, G Kowalski, M Burch, P Sepulveda, S Risis, R Lee-Young, S Lamon, P Meikle, A Genders, S McGee, M Watt, A Russell, M Frank, S Jackowski, M Febbraio, C Bruce

(2015), Vol. 21, pp. 718-730, Cell Metabolism, United States, C1

journal article

In vivo cardiac glucose metabolism in the high-fat fed mouse: comparison of euglycemic-hyperinsulinemic clamp derived measures of glucose uptake with a dynamic metabolomic flux profiling approach

G Kowalski, D De Souza, S Risis, M Burch, S Hamley, J Kloehn, A Selathurai, R Lee-Young, D Tull, S O'Callaghan, M McConville, C Bruce

(2015), Vol. 463, pp. 818-824, Biochemical and biophysical research communications, Amsterdam, The Netherlands, C1

journal article
2014

The regulation of glucose metabolism: implications and considerations for the assessment of glucose homeostasis in rodents

G Kowalski, C Bruce

(2014), Vol. 307, pp. E859-E871, American journal of physiology-endocrinology and metabolism, Bethesda, MD, C1

journal article

PLIN5 deletion remodels intracellular lipid composition and causes insulin resistance in muscle

Rachael Mason, Ruzaidi Mokhtar, Maria Matzaris, Ahrathy Selathurai, Greg Kowalski, Nancy Mokbel, Peter Meikle, Clinton Bruce, Matthew Watt

(2014), Vol. 3, pp. 652-663, Molecular metabolism, Amsterdam, Netherlands, C1-1

journal article
2013

Plasma sphingosine-1-phosphate is elevated in obesity

G Kowalski, A Carey, A Selathurai, B Kingwell, C Bruce

(2013), Vol. 8, pp. 1-7, PloS one, San Francisco, Calif., C1-1

journal article

Distinct patterns of tissue-specific lipid accumulation during the induction of insulin resistance in mice by high-fat feeding

N Turner, G Kowalski, S Leslie, S Risis, C Yang, R Lee-Young, J Babb, P Meikle, G Lancaster, D Henstridge, P White, E Kraegen, A Marette, G Cooney, M Febbraio, C Bruce

(2013), Vol. 56, pp. 1638-1648, Diabetologia, C1-1

journal article
2012

Overexpression of sphingosine kinase 1 prevents ceramide accumulation and ameliorates muscle insulin resistance in high-fat diet-fed mice

C Bruce, S Risis, J Babb, C Yang, G Kowalski, A Selathurai, R Lee-Young, J Weir, K Yoshioka, Y Takuwa, P Meikle, S Pitson, M Febbraio

(2012), Vol. 61, pp. 3148-3155, Diabetes, C1-1

journal article

Tumor progression locus 2 (Tpl2) deficiency does not protect against obesity-induced metabolic disease

G Lancaster, G Kowalski, E Estevez, M Kraakman, G Grigoriadis, M Febbraio, S Gerondakis, A Banerjee

(2012), Vol. 7, PLoS ONE, C1-1

journal article
2011

Deficiency of haematopoietic-cell-derived IL-10 does not exacerbate high-fat-diet-induced inflammation or insulin resistance in mice

G Kowalski, H Nicholls, S Risis, N Watson, P Kanellakis, C Bruce, A Bobik, G Lancaster, M Febbraio

(2011), Vol. 54, pp. 888-899, Diabetologia, C1-1

journal article

Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissue

H Nicholls, G Kowalski, D Kennedy, S Risis, L Zaffino, N Watson, P Kanellakis, M Watt, A Bobik, A Bonen, M Febbraio, G Lancaster, M Febbraio

(2011), Vol. 60, pp. 1100-1110, Diabetes, C1-1

journal article

IL-10 controls cystatin C synthesis and blood concentration in response to inflammation through regulation of IFN regulatory factor 8 expression

Y Xu, P Schnorrer, A Proietto, G Kowalski, M Febbraio, H Acha-Orbea, R Dickins, J Villadangos

(2011), Vol. 186, pp. 3666-3673, Journal of Immunology, C1-1

journal article

Funded Projects at Deakin

Australian Competitive Grants

Targeting hepatic energy expenditure to improve glycaemic control

Dr Greg Kowalski, A/Prof Clinton Bruce

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2015: $30,000
  • 2014: $30,000

Does mitochondrial dysfunction impair muscle glucose metabolism?

A/Prof Clinton Bruce, Dr Greg Kowalski, Dr Gunveen Kaur

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2015: $60,000

Liver-type pyruvate kinase: a novel anti-hyperglycemic target

Dr Greg Kowalski, A/Prof Clinton Bruce

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2016: $59,950

Phosphatidylserine: a regulator of muscle and mitochondrial biology?

Dr Greg Kowalski

ARC DECRA - Discovery Early Career Researcher Award

  • 2020: $130,507
  • 2019: $127,465
  • 2018: $125,192

Mechanisms of insulin resistance in humans

A/Prof Clinton Bruce, Dr Greg Kowalski

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2019: $60,000

Metabolic tug-of-war: deciphering the role of glucagon and insulin under physiologically relevant conditions in humans

Dr Greg Kowalski, A/Prof Clinton Bruce

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2019: $59,900

Aminogenic insulin-glucagon secretion and action in human insulin resistance

Dr Greg Kowalski, A/Prof Clinton Bruce

DART Grant - Research - Diabetes Australia - Victoria (Research Trust)

  • 2020: $60,000

Fasting hyperinsulinemia remodels adipose tissue metabolism to drive obesity and insulin resistance

Dr Chris Shaw, A/Prof Clinton Bruce, Dr Kirsten Howlett, Prof Sean Mcgee, Dr Greg Kowalski

Diabetes Australia Research Program Grant

  • 2021: $59,895

Industry and Other Funding

Determination of 6,6-2H glucose enrichment in plasma

A/Prof Clinton Bruce, Dr Greg Kowalski

  • 2015: $1,920

Measurement of plasma free fatty acids

A/Prof Clinton Bruce, Dr Greg Kowalski

  • 2016: $2,200

Other Funding Sources

Determination of 6,6-2H glucose enrichment in plasma

A/Prof Clinton Bruce, Dr Greg Kowalski

  • 2017: $776
  • 2016: $1,520
  • 2015: $1,710

Determination of lipogenesis in liver cancer

A/Prof Clinton Bruce, Dr Greg Kowalski

  • 2016: $4,000

Supervisions

Associate Supervisor
2021

Steven Douglas Hamley

Thesis entitled: Understanding the Mechanisms of Hyperinsulinemia

Doctor of Philosophy (Nutrition & Exercise) (High Cost), School of Exercise and Nutrition Sciences

2018

Dale John Morrison

Thesis entitled: Quantification of postprandial glucose flux following endurance exercise training and overfeeding by the triple tracer technique

Doctor of Philosophy (Nutrition & Exercise) (High Cost), School of Exercise and Nutrition Sciences