Prof John Stambas



Professor of Viral Immunology


Faculty of Health


School of Medicine


Geelong Waurn Ponds Campus


Graduate Certificate of Higher Education, Deakin University, 2012
Doctor of Philosophy, University of Melbourne, 2001
Bachelor of Science, University of Melbourne, 1996

+61 3 522 72487

Research interests

Research in Dr Stambass laboratory is primarily focused on understanding immune responses following virus infection and vaccination. The main scientific aim is to carefully dissect immune responses following infection (influenza virus-including H5N1 and pandemic H1N1 2009) and vaccination (HIV vaccine candidates) to determine relevant immune correlates of protection in order to drive innovative strategies for future vaccine development. Dr Stambas has made a significant contribution to his field as evidenced by publication history (27 peer-reviewed articles to date) and his invitations to speak at national and international conferences.


Honorary Senior Fellow, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia, 3010. (Nov 2009-present)

Teaching interests

A/Prof. Stambas serves as the Course Director for the Honours Program at the School of Medicine. A/Prof. Stambas supervises honours and PhD students.

Units taught

HMM104, HMH 402, HMO 303

Knowledge areas

immunology, virology, adaptive immunity, influenza virus, emerging zoonotic viruses, T cells, reverse genetics

Professional activities

Australasian Society for Immunology (ASI); 1996-present

American Association of Immunologists (AAI); 2003-present

Australasian Society for Immunology Treasurer and Executive member; 2013-2015

Member of the Safeguarding Australia Expert Working Group, developing a Strategic Roadmap for Australian Research Infrastructure for the Department of Innovation, Industry, Science and Research, Australian Government; 2011

Immunology Group of Victoria (IgV) committee, local branch of the Australasian Society for Immunology (ASI); 2008-2012

Plos One, Academic Editor; 2012-present


Dr Stambas has been awarded multiple research grants from local, national and international competitive schemes. These include three NHMRC grants as CI, an NHMRC Strategic Award for H1N1 Medical Research, an Australian Centre for HIV and Hepatitis Virology Research grant and a Bill and Melinda Gates Foundation Grand Challenges Exploration Grant. Dr Stambas has also received the highly competitive C.R. Roper Fellowship at the university of Melbourne and was awarded the Gordon Ada award from the Australasian Society for Immunology in 2012.


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CXCR6-deficiency improves the control of pulmonary Mycobacterium tuberculosis and influenza infection independent of T-Lymphocyte recruitment to the lungs

Anneliese Ashhurst, Manuela Flrido, Leon Lin, Diana Quan, Ellis Armitage, Sebastian Stifter, John Stambas, Warwick Britton

(2019), Vol. 10, pp. 1-16, Frontiers in immunology, Lausanne, Switzerland, C1


Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames

Damien Zanker, Sara Oveissi, David Tscharke, Mubing Duan, Siyuan Wan, Xiaomu Zhang, Kun Xiao, Nicole Mifsud, James Gibbs, Lenny Izzard, Daniel Dlugolenski, Pierre Faou, Karen Laurie, Nathalie Vigneron, Ian Barr, John Stambas, Benot Van den Eynde, Jack Bennink, Jonathan Yewdell, Weisan Chen

(2019), Vol. 202, pp. 3370-3380, Journal of immunology, Bethesda, Md., C1


Visualizing the Selectivity and Dynamics of Interferon Signaling In Vivo

S Stifter, N Bhattacharyya, A Sawyer, T Cootes, J Stambas, S Doyle, L Feigenbaum, W Paul, W Britton, A Sher, C Feng

(2019), Vol. 29, pp. 3539-3550.e4, Cell Reports, C1


Sequential pulmonary immunization with heterologous recombinant influenza A virus tuberculosis vaccines protects against murine M. tuberculosis infection

H Muflihah, M Flrido, L Lin, Y Xia, J Triccas, J Stambas, W Britton

(2018), Vol. 36, pp. 2462-2470, Vaccine, Amsterdam, The Netherlands, C1


Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens

Jasmina Luczo, Mary Tachedjian, Jennifer Harper, Jean Payne, Jeffrey Butler, Sandra Sapats, Suzanne Lowther, Wojtek Michalski, John Stambas, John Bingham

(2018), Vol. 8, pp. 1-13, Scientific reports, London, Eng., C1


Pulmonary immunization with a recombinant influenza A virus vaccine induces lung-resident CD4+ memory T cells that are associated with protection against tuberculosis

Manuela Flrido, Heni Muflihah, Leon Lin, Yingju Xia, Frederic Sierro, Mainthan Palendira, Carl Feng, Patrick Bertolino, John Stambas, James Triccas, Warwick Britton

(2018), Vol. 11, pp. 1743-1752, Mucosal immunology, London, Eng., C1


Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1

Siying Ye, Christopher Cowled, Cheng-Hon Yap, John Stambas

(2018), Vol. 8, Scientific reports, London, Eng., C1


Enhanced immunogenicity following miR-155 incorporation into the influenza A virus genome

L Izzard, D Dlugolenski, Y Xia, M McMahon, D Middleton, R Tripp, J Stambas

(2017), Vol. 235, pp. 115-120, Virus research, Amsterdam, The Netherlands, C1


Recombinant influenza virus expressing HIV-1 p24 capsid protein induces mucosal HIV-specific CD8 T-cell responses

H-X Tan, B Gilbertson, S Jegaskanda, S Alcantara, T Amarasena, J Stambas, J McAuley, S Kent, R De Rose

(2016), Vol. 34, pp. 1172-1179, Vaccine, Amsterdam, The Netherlands, C1


ADAMTS5 is a critical regulator of virus-specific T cell immunity

M McMahon, S Ye, L Izzard, D Dlugolenski, R Tripp, A Bean, D McCulloch, J Stambas

(2016), Vol. 14, pp. 1-22, PLoS biology, San Francisco, Calif., C1


Inhibition of reactive oxygen species production ameliorates inflammation induced by influenza A viruses via upregulation of SOCS1 and SOCS3.

S Ye, S Lowther, J Stambas

(2015), Vol. 89, pp. 2672-2683, Journal of Virology, United States, C1


Epitope-specific CD4+, but not CD8+, T-cell responses induced by recombinant influenza A viruses protect against Mycobacterium tuberculosis infection

M Flrido, R Pillay, C Gillis, Y Xia, S Turner, J Triccas, J Stambas, W Britton

(2015), Vol. 45, pp. 780-793, European journal of immunology, Weinheim, Germany, C1


Molecular pathogenesis of H5 highly pathogenic avian influenza: the role of the haemagglutinin cleavage site motif.

J Luczo, J Stambas, P Durr, W Michalski, J Bingham

(2015), Vol. 25, pp. 406-430, Reviews in medical virology, London, Eng., C1


Influenza reverse genetics: dissecting immunity and pathogenesis

S Ye, J Evans, J Stambas

(2014), Vol. 16, pp. 1-19, Expert reviews in molecular medicine, Cambridge, Eng., C1


miRNA modulation of SOCS1 using an influenza A virus delivery system

L Izzard, S Ye, K Jenkins, Y Xia, M Tizard, J Stambas

(2014), Vol. 95, pp. 1880-1885, Journal of general virology, Reading, England, C1


Apocynin inhibits reactive oxygen species (ROS) and downregulates chemokine and cytokine production induced by avian influenza H5N1and H7N9 virus infection

J Stambas, S Lowther, S Ye

(2014), Vol. 192, pp. 1-1, Journal of immunology, Bethesda, Md., C1-1


Glutathione peroxidase-1 reduces influenza A virus-induced lung inflammation

S Yatmaz, H Seow, R Gualano, Z Wong, J Stambas, S Selemidis, P Crack, S Bozinovski, G Anderson, R Vlahos

(2013), Vol. 48, pp. 17-26, American Journal of Respiratory Cell and Molecular Biology, C1


Trivalent live attenuated influenza-simian immunodeficiency virus vaccines: efficacy and evolution of cytotoxic T lymphocyte escape in macaques.

J Reece, S Alcantara, S Gooneratne, S Jegaskanda, T Amaresena, C Fernandez, K Laurie, A Hurt, S O'Connor, M Harris, J Petravic, A Martyushev, A Grimm, M Davenport, J Stambas, R De Rose, S Kent

(2013), Vol. 87, pp. 4146-4160, Journal of virology, Washington, D. C., C1


Promotion of Hendra virus replication by microRNA 146a

C Stewart, G Marsh, K Jenkins, M Gantier, M Tizard, D Middleton, J Lowenthal, J Haining, L Izzard, T Gough, C Deffrasnes, J Stambas, R Robinson, H Heine, J Pallister, A Foord, A Bean, L Wang

(2013), Vol. 87, pp. 3782-3791, Journal of virology, Washington, D. C., C1


Unique IL-13R?2-based HIV-1 vaccine strategy to enhance mucosal immunity, CD8(+) T-cell avidity and protective immunity

C Ranasinghe, S Trivedi, J Stambas, R Jackson

(2013), pp. 1-13, Mucosal immunology, London, England, C1


Influenza A virus infection impairs mycobacteria-specific T cell responses and mycobacterial clearance in the lung during pulmonary coinfection

M Flrido, M Grima, C Gillis, Y Xia, S Turner, J Triccas, J Stambas, W Britto

(2013), Vol. 191, pp. 302-311, Journal of Immunology, C1


Comparison of influenza and SIV specific CD8 T cell responses in macaques

S Jegaskanda, J Reece, R De Rose, J Stambas, L Sullivan, A Brooks, S Kent, A Sexton

(2012), Vol. 7, pp. 1-16, PLoS one, San Francisco, CA, C1-1


A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus-poxvirus prime boost immunisations

C Ranasinghe, F Eyers, J Stambas, D Boyle, I Ramshaw, A Ramsay

(2011), Vol. 29, pp. 3008-3020, Vaccine, C1-1


Inhibition of Nox2 oxidase activity ameliorates influenza A virus-induced lung inflammation

R Vlahos, J Stambas, S Bozinovski, B Broughton, G Drummond, S Selemidis

(2011), Vol. 7, pp. 1-12, PLoS Pathogens, San Francisco, Calif., C1


A Gel-capture assay for characterizing the sialyl-glycan selectivity of influenza viruses

T Velkov, P Thompson, O El-Kabbani, F Lindh, J Stambas, S Rockman

(2011), Vol. 55, pp. 131-137, Acta Virologica, C1


Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

K Kedzierska, S Valkenburg, C Guillonneau, F Hubert, T Cukalac, J Curtis, J Stambas, H Scott, L Kedzierski, V Venturi, M Davenport

(2010), Vol. 40, pp. 849-858, European Journal of Immunology, C1-1


Influenza a virus-specific CD8+ T-cell responses: From induction to function

M Olson, B Russ, P Doherty, S Turner, J Stambas

(2010), Vol. 5, pp. 175-183, Future Virology, C1


Timing of immune escape linked to success or failure of vaccination

J Reece, L Loh, S Alcantara, C Fernandez, J Stambas, A Sexton, R De Rose, J Petravic, M Davenport, S Kent

(2010), Vol. 5, pp. 1-7, Plos one, San Francisco, Calif., C1-1


Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env311-320 virus

T Cukalac, J Moffat, V Venturi, M Davenport, P Doherty, S Turner, J Stambas

(2009), Vol. 27, pp. 6755-6761, Vaccine, C1-1


Evaluation of recombinant influenza virus-simian immunodeficiency virus vaccines in macaques

A Sexton, R De Rose, J Reece, S Alcantara, L Loh, J Moffat, K Laurie, A Hurt, P Doherty, S Turner, S Kent, J Stambas

(2009), Vol. 83, pp. 7619-7628, Journal of virology, Washington, DC, C1-1


HIV-1 vaccine development: Tackling virus diversity with a multi-envelope cocktail

J Hurwitz, X Zhan, S Brown, M Bonsignori, J Stambas, T Lockey, R Sealy, S Surman, P Freiden, B Jones, L Martin, J Blanchard, K Slobod

(2008), Vol. 13, pp. 609-620, Frontiers in Bioscience, C1-1


Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity

K Kedzierska, N La Gruta, J Stambas, S Turner, P Doherty

(2008), Vol. 45, pp. 607-618, Molecular Immunology, C1-1


Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination

A Miller, R Center, J Stambas, G Deliyannis, P Doherty, J Howard, S Turner, D Purcell

(2008), Vol. 26, pp. 5641-5648, Vaccine, C1-1


Killer T cells in influenza

J Stambas, C Guillonneau, K Kedzierska, J Mintern, P Doherty, N La Gruta

(2008), Vol. 120, pp. 186-196, Pharmacology and Therapeutics, C1-1


Location rather than CD62L phenotype is critical in the early establishment of influenza-specific CD8+ T cell memory

K Kedzierska, J Stambas, M Jenkins, R Keating, S Turner, P Doherty

(2007), Vol. 104, pp. 9782-9787, Proceedings of the National Academy of Sciences of the United States of America, C1-1


A question of self-preservation: Immunopathology in influenza virus infection

N La Gruta, K Kedzierska, J Stambas, P Doherty

(2007), Vol. 85, pp. 85-92, Immunology and Cell Biology, C1-1


An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells

J Stambas, P Doherty, S Turner

(2007), Vol. 178, pp. 1285-1292, Journal of Immunology, C1-1


A multi-vector, multi-envelope HIV-1 vaccine

J Hurwitz, X Zhan, S Brown, M Bonsignori, J Stambas, T Lockey, B Jones, S Surman, R Sealy, P Freiden, K Branum, K Slobod

(2007), Vol. 12, pp. 68-76, The Journal of pediatric pharmacology and therapeutics, Littleton, Colorado, C1-1


HIV vaccines: Brief review and discussion of future directions

K Slobod, M Bonsignori, S Brown, X Zhan, J Stambas, J Hurwitz

(2005), Vol. 4, pp. 305-313, Expert Review of Vaccines, C1-1


HIV vaccine rationale, design and testing

K Slobod, C Coleclough, M Bonsignori, S Brown, X Zhan, S Surman, A Zirkel, B Jones, R Sealy, J Stambas, B Brown, T Lockey, P Freiden, P Doherty, J Blanchard, L Martin, J Hurwitz

(2005), Vol. 3, pp. 107-112, Current HIV Research, C1-1


Clade, country and region-specific HIV-1 vaccines : are they necessary?

K Slobod, C Coleclough, S Brown, J Stambas, X Zhan, S Surman, B Jones, A Zirkel, P Freiden, B Brown, R Sealy, M Bonsignori, J Hurwitz

(2005), Vol. 2, pp. 1-3, AIDS Research and Therapy, London, England, C1-1


Consequences of immunodominant epitope deletion for minor influenza virus-specific CD8+-T-cell responses

S Andreansky, J Stambas, P Thomas, W Xie, R Webby, P Doherty

(2005), Vol. 79, pp. 4329-4339, Journal of Virology, Washington, D.C., C1-1


Long lived multi-isotype anti-HIV antibody responses following a prime-double boost immunization strategy

J Stambas, S Brown, A Gutierrez, R Sealy, W Yue, B Jones, T Lockey, A Zirkel, P Freiden, B Brown, S Surman, C Coleclough, K Slobod, P Doherty, J Hurwitz

(2005), Vol. 23, pp. 2454-2464, Vaccine, C1-1


Multi-envelope HIV-1 vaccine devoid of SIV components controls disease in macaques challenged with heterologous pathogenic SHIV

X Zhan, L Martin, K Slobod, C Coleclough, T Lockey, S Brown, J Stambas, M Bonsignori, R Sealy, J Blanchard, J Hurwitz

(2005), Vol. 23, pp. 5306-5320, Vaccine, C1-1


Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

S Andreansky, J Stambas, A Gutierrez, G Diaz, P Doherty, S Turner, R Webby

(2004), Vol. 1263, pp. 141-144, International Congress Series, C1-1


Differential tumor necrosis factor receptor 2-mediated editing of virus-specific CD8+ effector T cells

S Turner, N La Gruta, J Stambas, G Diaz, P Doherty

(2004), Vol. 101, pp. 3545-3550, Proceedings of the National Academy of Sciences of the United States of America, C1-1


Clustering of Th cell epitopes on exposed regions of HIV envelope despite defects in antibody activity

S Brown, J Stambas, X Zhan, K Slobod, C Coleclough, A Zirkel, S Surman, S White, P Doherty, J Hurwitz

(2003), Vol. 171, pp. 4140-4148, Journal of Immunology, C1-1


Protection and compensation in the influenza virus-specific CD8+ T cell response

R Webby, S Andreanskyt, J Stambast, J Rehg, R Webster, P Doherty, S Turner

(2003), Vol. 100, pp. 7235-7240, Proceedings of the National Academy of Sciences of the United States of America, C1-1


Oxidised mannan as a novel adjuvant inducing mucosal IgA production

J Stambas, G Pietersz, I McKenzie, C Cheers

(2002), Vol. 20, pp. 1068-1078, Vaccine, C1-1


Oxidised mannan-listeriolysin O conjugates induce Th1/Th2 cytokine responses after intranasal immunisation

J Stambas, G Pietersz, I McKenzie, V Nagabhushanam, C Cheers

(2002), Vol. 20, pp. 1877-1886, Vaccine, C1-1


Funded Projects at Deakin

Australian Competitive Grants

Identification of determinants associated with pathogenicity of Swine-Origin 2009 (H1N1) influenza virus

Prof John Stambas, A/Prof Stephen Stephen, Dr Deborah Middleton

NHMRC - H1N1 Influenza 09

  • 2011: $30,809
  • 2010: $68,985

Evaluation of immune correlates for virus-specific CD8+ T cells following prime-boost vaccination

Prof John Stambas

NHMRC Project Grant

  • 2011: $2,064
  • 2010: $159,691

Development of recombinant influenza-SIV vaccines

Prof John Stambas, Dr Robert De Rose

University of Melbourne - Aust Centre for HIV and Hepatitis Virology Research

  • 2009: $66,000

Comparative effectiveness of vaccine-induced SIV-specific CD8 T cells

Prof Steven Kent, Prof John Stambas

NHMRC Project Grant

  • 2012: $90,000
  • 2011: $90,000

Understanding immunity in an encephalitic mouse model of Hendra virus infection

Prof John Stambas, Prof Paul Hertzog, Prof Carola Vinuesa, Dr Deborah Middleton, Dr Linfa Wang

NHMRC Hendra Virus Urgent Call for Research

  • 2014: $90,600
  • 2013: $28,305
  • 2012: $187,450

Impact of Influenza A infection on T cell-mediated immunity to pulmonary tuberculosis

Prof Warwick Britton, Dr Jamie Triccas, Dr Manuela Florido, Prof John Stambas

NHMRC Project Grant

  • 2013: $25,000

Development of apocynin as a new antiviral for emerging infectious diseases

Prof John Stambas

CSIRO Flagship Collaboration Research Fund - Commonwealth Scientific and Industrial Research Organisation

  • 2013: $85,000

The role of extracellular matrix enzymes in influenza virus infection

Prof John Stambas, Prof Ralph Tripp, Prof Suneel Apte

NHMRC Project Grant

  • 2020: $78,030
  • 2019: $118,533

Other Public Sector Funding

The role of endothelium in the pathogenicity of H5N1 HPAI in chickens

Prof John Stambas, Ms Jasmina Luczo

  • 2014: $14,000
  • 2013: $28,000
  • 2012: $42,000
  • 2011: $14,000

Design and development of superior pan-diagnostics for Foot-and-Mouth Disease (FMD)

Prof John Stambas, Miss Alyssa Van Dreumel

  • 2015: $2,136
  • 2014: $4,272
  • 2013: $4,272
  • 2012: $4,272

Delivery of MIRNA Using Genetically Modified Influenza Virus

Prof John Stambas, Prof Alister Ward, Prof Ken Walder, Ms Yingju Xia

  • 2014: $60,000

Industry and Other Funding

Generation of recombinant influenza vaccines expressing exogenous miRNAs for increased in vitro vaccine production

Prof Ralph Tripp, Prof John Stambas

  • 2019: $8,600
  • 2018: $30,268
  • 2017: $24,823

CISH (cytokine-inducible SH2-containing protein): a biological universal remote for CD8+ T cell regulation

Prof John Stambas, Dr Daniel Dlugolenski

  • 2018: $31,950
  • 2017: $26,101

Other Funding Sources

Collaborative High Bio-Containment immunological Research Facility

Prof Alister Ward, Dr Linfa Wang, Prof John Stambas, Dr John Lowenthal, Prof Ralph Tripp, Prof Johnson Mak, Prof Bill Buttemer, Prof Tania De Koning-Ward

  • 2012: $105,000
  • 2011: $100,000


Principal Supervisor

Qi Liu

Thesis entitled: Gene editing in chickens : Knockdown of Interleukin-6 using CRISPR

Doctor of Philosophy (Medicine), School of Medicine


Yingju Xia

Thesis entitled: Delivery of Immunomodulatory miRNAs Using Genetically Modified Influenza Viruses

Doctor of Philosophy (Medicine), School of Medicine

Meagan McMahon

Thesis entitled: The role of ADAMTS enzymes in influenza virus immunity

Doctor of Philosophy (Medicine), School of Medicine


Jasmina Luczo

Thesis entitled: Pathogenesis of avian influenza: role of the haemagglutinin cleavage site motif

Doctor of Philosophy (Medicine), School of Medicine

Alyssa Van Dreumel

Thesis entitled: Design and development of Pan-serodiagnostics for Foot-and-mouth disease

Doctor of Philosophy (Medicine), School of Medicine


Mohammed Jasim Mohammed Shallal

Thesis entitled: Recombinant Influenza virus as HIV vaccine vector

Doctor of Philosophy (Medicine), School of Medicine

Associate Supervisor

Corinna Pade

Thesis entitled: Dissecting the early entry steps of HIV-1 infection

Doctor of Philosophy (Medicine), School of Medicine


Brian Clarke

Thesis entitled: Expression of anti-viral shRNA molecules in transgenic zebrafish

Doctor of Philosophy (Medicine), School of Medicine


Katherine Birrer

Thesis entitled: Silencing Host Genes Using RNAi to Impact Virus Growth in vitro Methods for reducing cotton wax.

MAppSc (Medicine) (High cost), School of Medicine