Prof John Stambas

STAFF PROFILE

Position

Professor of Viral Immunology

Faculty

Faculty of Health

Department

School of Medicine

Campus

Geelong Waurn Ponds Campus

Qualifications

Graduate Certificate of Higher Education, Deakin University, 2012
Doctor of Philosophy, University of Melbourne, 2001
Bachelor of Science, University of Melbourne, 1996

Contact

john.stambas@deakin.edu.au
+61 3 522 72487

Research interests

Research in the Stambas laboratory is primarily focused on understanding immune responses following virus infection and vaccination. The main scientific aim is to carefully dissect immune responses following virus infection and vaccination to determine relevant immune correlates of protection in order to drive innovative strategies for future vaccine or therapeutic development. Prof. Stambas has made a significant contribution to his field as evidenced by his publication history and his invitations to organise and present at local, national and international conferences.

Affiliations

Honorary Senior Fellow, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia, 3010. (Nov 2009-present)

Teaching interests

Prof. Stambas undertsands the importance of training the next generation of scientists, science educators or health  professionals and as such teaches immunology to undergraduate students in the Bachelor of Biomedical Science degree at Deakin University.

Prof. Stambas served as Course Director for the School of Medicine Honours Program between 2011-2019 and in 2020 was appointed HDR coordinator in the school. He trains honours, M Phil and PhD students in his laboratory.

Units taught

HMM204 and HMO 303

Knowledge areas

immunology, virology, adaptive and innate immunity, influenza virus and T cells

Professional activities

Australasian Society for Immunology (ASI); 1996-present

American Association of Immunologists (AAI); 2003-present

Australasian Society for Immunology Treasurer and Executive member; 2013-2015

Member of the Safeguarding Australia Expert Working Group, developing a Strategic Roadmap for Australian Research Infrastructure for the Department of Innovation, Industry, Science and Research, Australian Government; 2011

Immunology Group of Victoria (IgV) committee, local branch of the Australasian Society for Immunology (ASI); 2008-2012

Plos One, Academic Editor; 2012-present

Awards

Prof. Stambas has been awarded numerous competitive research and travel grants from local, national and international funding agencies including the NHMRC, ARC and AAI. Prof. Stambas has previously received a C.R. Roper Fellowship to undertake post-doctoral training at the University of Melbourne and was awarded a Gordon Ada Senior Travel Award from the Australian and New Zealand Society for Immunology.

Publications

Filter by

2019

CXCR6-deficiency improves the control of pulmonary Mycobacterium tuberculosis and influenza infection independent of T-Lymphocyte recruitment to the lungs

Anneliese Ashhurst, Manuela Flrido, Leon Lin, Diana Quan, Ellis Armitage, Sebastian Stifter, John Stambas, Warwick Britton

(2019), Vol. 10, pp. 1-16, Frontiers in immunology, Lausanne, Switzerland, C1

journal article

Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames

Damien Zanker, Sara Oveissi, David Tscharke, Mubing Duan, Siyuan Wan, Xiaomu Zhang, Kun Xiao, Nicole Mifsud, James Gibbs, Lenny Izzard, Daniel Dlugolenski, Pierre Faou, Karen Laurie, Nathalie Vigneron, Ian Barr, John Stambas, Benot Van den Eynde, Jack Bennink, Jonathan Yewdell, Weisan Chen

(2019), Vol. 202, pp. 3370-3380, Journal of immunology, Bethesda, Md., C1

journal article

Visualizing the Selectivity and Dynamics of Interferon Signaling In Vivo

S Stifter, N Bhattacharyya, A Sawyer, T Cootes, J Stambas, S Doyle, L Feigenbaum, W Paul, W Britton, A Sher, C Feng

(2019), Vol. 29, pp. 3539-3550, Cell Reports, Cambridge, Ma., C1

journal article
2018

Sequential pulmonary immunization with heterologous recombinant influenza A virus tuberculosis vaccines protects against murine M. tuberculosis infection

H Muflihah, M Flrido, L Lin, Y Xia, J Triccas, J Stambas, W Britton

(2018), Vol. 36, pp. 2462-2470, Vaccine, Amsterdam, The Netherlands, C1

journal article

Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens

Jasmina Luczo, Mary Tachedjian, Jennifer Harper, Jean Payne, Jeffrey Butler, Sandra Sapats, Suzanne Lowther, Wojtek Michalski, John Stambas, John Bingham

(2018), Vol. 8, pp. 1-13, Scientific reports, London, Eng., C1

journal article

Pulmonary immunization with a recombinant influenza A virus vaccine induces lung-resident CD4+ memory T cells that are associated with protection against tuberculosis

Manuela Flrido, Heni Muflihah, Leon Lin, Yingju Xia, Frederic Sierro, Mainthan Palendira, Carl Feng, Patrick Bertolino, John Stambas, James Triccas, Warwick Britton

(2018), Vol. 11, pp. 1743-1752, Mucosal immunology, London, Eng., C1

journal article

Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1

Siying Ye, Christopher Cowled, Cheng-Hon Yap, John Stambas

(2018), Vol. 8, Scientific reports, London, Eng., C1

journal article
2017

Enhanced immunogenicity following miR-155 incorporation into the influenza A virus genome

L Izzard, D Dlugolenski, Y Xia, M McMahon, D Middleton, R Tripp, J Stambas

(2017), Vol. 235, pp. 115-120, Virus research, Amsterdam, The Netherlands, C1

journal article
2016

Recombinant influenza virus expressing HIV-1 p24 capsid protein induces mucosal HIV-specific CD8 T-cell responses

H-X Tan, B Gilbertson, S Jegaskanda, S Alcantara, T Amarasena, J Stambas, J McAuley, S Kent, R De Rose

(2016), Vol. 34, pp. 1172-1179, Vaccine, Amsterdam, The Netherlands, C1

journal article

ADAMTS5 is a critical regulator of virus-specific T cell immunity

M McMahon, S Ye, L Izzard, D Dlugolenski, R Tripp, A Bean, D McCulloch, J Stambas

(2016), Vol. 14, pp. 1-22, PLoS biology, San Francisco, Calif., C1

journal article
2015

Inhibition of reactive oxygen species production ameliorates inflammation induced by influenza A viruses via upregulation of SOCS1 and SOCS3.

S Ye, S Lowther, J Stambas

(2015), Vol. 89, pp. 2672-2683, Journal of Virology, United States, C1

journal article

Epitope-specific CD4+, but not CD8+, T-cell responses induced by recombinant influenza A viruses protect against Mycobacterium tuberculosis infection

M Flrido, R Pillay, C Gillis, Y Xia, S Turner, J Triccas, J Stambas, W Britton

(2015), Vol. 45, pp. 780-793, European journal of immunology, Weinheim, Germany, C1

journal article

Molecular pathogenesis of H5 highly pathogenic avian influenza: the role of the haemagglutinin cleavage site motif.

J Luczo, J Stambas, P Durr, W Michalski, J Bingham

(2015), Vol. 25, pp. 406-430, Reviews in medical virology, London, Eng., C1

journal article
2014

Influenza reverse genetics: dissecting immunity and pathogenesis

S Ye, J Evans, J Stambas

(2014), Vol. 16, pp. 1-19, Expert reviews in molecular medicine, Cambridge, Eng., C1

journal article

miRNA modulation of SOCS1 using an influenza A virus delivery system

L Izzard, S Ye, K Jenkins, Y Xia, M Tizard, J Stambas

(2014), Vol. 95, pp. 1880-1885, Journal of general virology, Reading, England, C1

journal article

Apocynin inhibits reactive oxygen species (ROS) and downregulates chemokine and cytokine production induced by avian influenza H5N1and H7N9 virus infection

J Stambas, S Lowther, S Ye

(2014), Vol. 192, pp. 1-1, Journal of immunology, Bethesda, Md., C1-1

journal article
2013

Glutathione peroxidase-1 reduces influenza A virus-induced lung inflammation

S Yatmaz, H Seow, R Gualano, Z Wong, J Stambas, S Selemidis, P Crack, S Bozinovski, G Anderson, R Vlahos

(2013), Vol. 48, pp. 17-26, American Journal of Respiratory Cell and Molecular Biology, C1

journal article

Trivalent live attenuated influenza-simian immunodeficiency virus vaccines: efficacy and evolution of cytotoxic T lymphocyte escape in macaques.

J Reece, S Alcantara, S Gooneratne, S Jegaskanda, T Amaresena, C Fernandez, K Laurie, A Hurt, S O'Connor, M Harris, J Petravic, A Martyushev, A Grimm, M Davenport, J Stambas, R De Rose, S Kent

(2013), Vol. 87, pp. 4146-4160, Journal of virology, Washington, D. C., C1

journal article

Promotion of Hendra virus replication by microRNA 146a

C Stewart, G Marsh, K Jenkins, M Gantier, M Tizard, D Middleton, J Lowenthal, J Haining, L Izzard, T Gough, C Deffrasnes, J Stambas, R Robinson, H Heine, J Pallister, A Foord, A Bean, L Wang

(2013), Vol. 87, pp. 3782-3791, Journal of virology, Washington, D. C., C1

journal article

Unique IL-13R?2-based HIV-1 vaccine strategy to enhance mucosal immunity, CD8(+) T-cell avidity and protective immunity

C Ranasinghe, S Trivedi, J Stambas, R Jackson

(2013), pp. 1-13, Mucosal immunology, London, England, C1

journal article

Influenza A virus infection impairs mycobacteria-specific T cell responses and mycobacterial clearance in the lung during pulmonary coinfection

M Flrido, M Grima, C Gillis, Y Xia, S Turner, J Triccas, J Stambas, W Britto

(2013), Vol. 191, pp. 302-311, Journal of Immunology, C1

journal article
2012

Comparison of influenza and SIV specific CD8 T cell responses in macaques

S Jegaskanda, J Reece, R De Rose, J Stambas, L Sullivan, A Brooks, S Kent, A Sexton

(2012), Vol. 7, pp. 1-16, PLoS one, San Francisco, CA, C1-1

journal article
2011

A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus-poxvirus prime boost immunisations

C Ranasinghe, F Eyers, J Stambas, D Boyle, I Ramshaw, A Ramsay

(2011), Vol. 29, pp. 3008-3020, Vaccine, C1-1

journal article

Inhibition of Nox2 oxidase activity ameliorates influenza A virus-induced lung inflammation

R Vlahos, J Stambas, S Bozinovski, B Broughton, G Drummond, S Selemidis

(2011), Vol. 7, pp. 1-12, PLoS Pathogens, San Francisco, Calif., C1

journal article

A Gel-capture assay for characterizing the sialyl-glycan selectivity of influenza viruses

T Velkov, P Thompson, O El-Kabbani, F Lindh, J Stambas, S Rockman

(2011), Vol. 55, pp. 131-137, Acta Virologica, C1

journal article
2010

Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire

K Kedzierska, S Valkenburg, C Guillonneau, F Hubert, T Cukalac, J Curtis, J Stambas, H Scott, L Kedzierski, V Venturi, M Davenport

(2010), Vol. 40, pp. 849-858, European Journal of Immunology, C1-1

journal article

Influenza a virus-specific CD8+ T-cell responses: From induction to function

M Olson, B Russ, P Doherty, S Turner, J Stambas

(2010), Vol. 5, pp. 175-183, Future Virology, C1

journal article

Timing of immune escape linked to success or failure of vaccination

J Reece, L Loh, S Alcantara, C Fernandez, J Stambas, A Sexton, R De Rose, J Petravic, M Davenport, S Kent

(2010), Vol. 5, pp. 1-7, Plos one, San Francisco, Calif., C1-1

journal article
2009

Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env311-320 virus

T Cukalac, J Moffat, V Venturi, M Davenport, P Doherty, S Turner, J Stambas

(2009), Vol. 27, pp. 6755-6761, Vaccine, C1-1

journal article

Evaluation of recombinant influenza virus-simian immunodeficiency virus vaccines in macaques

A Sexton, R De Rose, J Reece, S Alcantara, L Loh, J Moffat, K Laurie, A Hurt, P Doherty, S Turner, S Kent, J Stambas

(2009), Vol. 83, pp. 7619-7628, Journal of virology, Washington, DC, C1-1

journal article
2008

HIV-1 vaccine development: Tackling virus diversity with a multi-envelope cocktail

J Hurwitz, X Zhan, S Brown, M Bonsignori, J Stambas, T Lockey, R Sealy, S Surman, P Freiden, B Jones, L Martin, J Blanchard, K Slobod

(2008), Vol. 13, pp. 609-620, Frontiers in Bioscience, C1-1

journal article

Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity

K Kedzierska, N La Gruta, J Stambas, S Turner, P Doherty

(2008), Vol. 45, pp. 607-618, Molecular Immunology, C1-1

journal article

Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination

A Miller, R Center, J Stambas, G Deliyannis, P Doherty, J Howard, S Turner, D Purcell

(2008), Vol. 26, pp. 5641-5648, Vaccine, C1-1

journal article

Killer T cells in influenza

J Stambas, C Guillonneau, K Kedzierska, J Mintern, P Doherty, N La Gruta

(2008), Vol. 120, pp. 186-196, Pharmacology and Therapeutics, C1-1

journal article
2007

Location rather than CD62L phenotype is critical in the early establishment of influenza-specific CD8+ T cell memory

K Kedzierska, J Stambas, M Jenkins, R Keating, S Turner, P Doherty

(2007), Vol. 104, pp. 9782-9787, Proceedings of the National Academy of Sciences of the United States of America, C1-1

journal article

A question of self-preservation: Immunopathology in influenza virus infection

N La Gruta, K Kedzierska, J Stambas, P Doherty

(2007), Vol. 85, pp. 85-92, Immunology and Cell Biology, C1-1

journal article

An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8+ T cells

J Stambas, P Doherty, S Turner

(2007), Vol. 178, pp. 1285-1292, Journal of Immunology, C1-1

journal article

A multi-vector, multi-envelope HIV-1 vaccine

J Hurwitz, X Zhan, S Brown, M Bonsignori, J Stambas, T Lockey, B Jones, S Surman, R Sealy, P Freiden, K Branum, K Slobod

(2007), Vol. 12, pp. 68-76, The Journal of pediatric pharmacology and therapeutics, Littleton, Colorado, C1-1

journal article
2005

HIV vaccines: Brief review and discussion of future directions

K Slobod, M Bonsignori, S Brown, X Zhan, J Stambas, J Hurwitz

(2005), Vol. 4, pp. 305-313, Expert Review of Vaccines, C1-1

journal article

HIV vaccine rationale, design and testing

K Slobod, C Coleclough, M Bonsignori, S Brown, X Zhan, S Surman, A Zirkel, B Jones, R Sealy, J Stambas, B Brown, T Lockey, P Freiden, P Doherty, J Blanchard, L Martin, J Hurwitz

(2005), Vol. 3, pp. 107-112, Current HIV Research, C1-1

journal article

Clade, country and region-specific HIV-1 vaccines : are they necessary?

K Slobod, C Coleclough, S Brown, J Stambas, X Zhan, S Surman, B Jones, A Zirkel, P Freiden, B Brown, R Sealy, M Bonsignori, J Hurwitz

(2005), Vol. 2, pp. 1-3, AIDS Research and Therapy, London, England, C1-1

journal article

Consequences of immunodominant epitope deletion for minor influenza virus-specific CD8+-T-cell responses

S Andreansky, J Stambas, P Thomas, W Xie, R Webby, P Doherty

(2005), Vol. 79, pp. 4329-4339, Journal of Virology, Washington, D.C., C1-1

journal article

Long lived multi-isotype anti-HIV antibody responses following a prime-double boost immunization strategy

J Stambas, S Brown, A Gutierrez, R Sealy, W Yue, B Jones, T Lockey, A Zirkel, P Freiden, B Brown, S Surman, C Coleclough, K Slobod, P Doherty, J Hurwitz

(2005), Vol. 23, pp. 2454-2464, Vaccine, C1-1

journal article

Multi-envelope HIV-1 vaccine devoid of SIV components controls disease in macaques challenged with heterologous pathogenic SHIV

X Zhan, L Martin, K Slobod, C Coleclough, T Lockey, S Brown, J Stambas, M Bonsignori, R Sealy, J Blanchard, J Hurwitz

(2005), Vol. 23, pp. 5306-5320, Vaccine, C1-1

journal article
2004

Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes

S Andreansky, J Stambas, A Gutierrez, G Diaz, P Doherty, S Turner, R Webby

(2004), Vol. 1263, pp. 141-144, International Congress Series, C1-1

journal article

Differential tumor necrosis factor receptor 2-mediated editing of virus-specific CD8+ effector T cells

S Turner, N La Gruta, J Stambas, G Diaz, P Doherty

(2004), Vol. 101, pp. 3545-3550, Proceedings of the National Academy of Sciences of the United States of America, C1-1

journal article
2003

Clustering of Th cell epitopes on exposed regions of HIV envelope despite defects in antibody activity

S Brown, J Stambas, X Zhan, K Slobod, C Coleclough, A Zirkel, S Surman, S White, P Doherty, J Hurwitz

(2003), Vol. 171, pp. 4140-4148, Journal of Immunology, C1-1

journal article

Protection and compensation in the influenza virus-specific CD8+ T cell response

R Webby, S Andreanskyt, J Stambast, J Rehg, R Webster, P Doherty, S Turner

(2003), Vol. 100, pp. 7235-7240, Proceedings of the National Academy of Sciences of the United States of America, C1-1

journal article
2002

Oxidised mannan as a novel adjuvant inducing mucosal IgA production

J Stambas, G Pietersz, I McKenzie, C Cheers

(2002), Vol. 20, pp. 1068-1078, Vaccine, C1-1

journal article

Oxidised mannan-listeriolysin O conjugates induce Th1/Th2 cytokine responses after intranasal immunisation

J Stambas, G Pietersz, I McKenzie, V Nagabhushanam, C Cheers

(2002), Vol. 20, pp. 1877-1886, Vaccine, C1-1

journal article

Funded Projects at Deakin

Australian Competitive Grants

Identification of determinants associated with pathogenicity of Swine-Origin 2009 (H1N1) influenza virus

Prof John Stambas, A/Prof Stephen Stephen, Dr Deborah Middleton

NHMRC - H1N1 Influenza 09

  • 2011: $30,809
  • 2010: $68,985

Evaluation of immune correlates for virus-specific CD8+ T cells following prime-boost vaccination

Prof John Stambas

NHMRC Project Grant

  • 2011: $2,064
  • 2010: $159,691

Development of recombinant influenza-SIV vaccines

Prof John Stambas, Dr Robert De Rose

University of Melbourne - Aust Centre for HIV and Hepatitis Virology Research

  • 2009: $66,000

Comparative effectiveness of vaccine-induced SIV-specific CD8 T cells

Prof Steven Kent, Prof John Stambas

NHMRC Project Grant

  • 2012: $90,000
  • 2011: $90,000

Understanding immunity in an encephalitic mouse model of Hendra virus infection

Prof John Stambas, Prof Paul Hertzog, Prof Carola Vinuesa, Dr Deborah Middleton, Dr Linfa Wang

NHMRC Hendra Virus Urgent Call for Research

  • 2014: $90,600
  • 2013: $28,305
  • 2012: $187,450

Impact of Influenza A infection on T cell-mediated immunity to pulmonary tuberculosis

Prof Warwick Britton, Dr Jamie Triccas, Dr Manuela Florido, Prof John Stambas

NHMRC Project Grant

  • 2013: $25,000

Development of apocynin as a new antiviral for emerging infectious diseases

Prof John Stambas

CSIRO Flagship Collaboration Research Fund - Commonwealth Scientific and Industrial Research Organisation

  • 2013: $85,000

The role of extracellular matrix enzymes in influenza virus infection

Prof John Stambas, Prof Ralph Tripp, Prof Suneel Apte

NHMRC Project Grant

  • 2020: $109,243
  • 2019: $118,533

Other Public Sector Funding

The role of endothelium in the pathogenicity of H5N1 HPAI in chickens

Prof John Stambas, Ms Jasmina Luczo

  • 2014: $14,000
  • 2013: $28,000
  • 2012: $42,000
  • 2011: $14,000

Design and development of superior pan-diagnostics for Foot-and-Mouth Disease (FMD)

Prof John Stambas, Miss Alyssa Van Dreumel

  • 2015: $2,136
  • 2014: $4,272
  • 2013: $4,272
  • 2012: $4,272

Delivery of MIRNA Using Genetically Modified Influenza Virus

Prof John Stambas, Prof Alister Ward, Prof Ken Walder, Ms Yingju Xia

  • 2014: $60,000

Industry and Other Funding

Generation of recombinant influenza vaccines expressing exogenous miRNAs for increased in vitro vaccine production

Prof Ralph Tripp, Prof John Stambas

  • 2019: $8,600
  • 2018: $30,268
  • 2017: $24,823

CISH (cytokine-inducible SH2-containing protein): a biological universal remote for CD8+ T cell regulation

Prof John Stambas, Dr Daniel Dlugolenski

  • 2018: $31,950
  • 2017: $26,101

Other Funding Sources

Collaborative High Bio-Containment immunological Research Facility

Prof Alister Ward, Dr Linfa Wang, Prof John Stambas, Dr John Lowenthal, Prof Ralph Tripp, Prof Johnson Mak, Prof Bill Buttemer, Prof Tania De Koning-Ward

  • 2012: $105,000
  • 2011: $100,000

Supervisions

Principal Supervisor
2018

Qi Liu

Thesis entitled: Gene editing in chickens : Knockdown of Interleukin-6 using CRISPR

Doctor of Philosophy (Medicine), School of Medicine

2017

Yingju Xia

Thesis entitled: Delivery of Immunomodulatory miRNAs Using Genetically Modified Influenza Viruses

Doctor of Philosophy (Medicine), School of Medicine

Meagan McMahon

Thesis entitled: The role of ADAMTS enzymes in influenza virus immunity

Doctor of Philosophy (Medicine), School of Medicine

2016

Jasmina Luczo

Thesis entitled: Pathogenesis of avian influenza: role of the haemagglutinin cleavage site motif

Doctor of Philosophy (Medicine), School of Medicine

Alyssa Van Dreumel

Thesis entitled: Design and development of Pan-serodiagnostics for Foot-and-mouth disease

Doctor of Philosophy (Medicine), School of Medicine

2015

Mohammed Jasim Mohammed Shallal

Thesis entitled: Recombinant Influenza virus as HIV vaccine vector

Doctor of Philosophy (Medicine), School of Medicine

Associate Supervisor
2016

Corinna Pade

Thesis entitled: Dissecting the early entry steps of HIV-1 infection

Doctor of Philosophy (Medicine), School of Medicine

2015

Brian Clarke

Thesis entitled: Expression of anti-viral shRNA molecules in transgenic zebrafish

Doctor of Philosophy (Medicine), School of Medicine

2012

Katherine Birrer

Thesis entitled: Silencing Host Genes Using RNAi to Impact Virus Growth in vitro Methods for reducing cotton wax.

MAppSc (Medicine) (High cost), School of Medicine