Dr Matthew McKenzie

STAFF PROFILE

Position

Lecturer in Animal Cell Biology

Faculty

Faculty of Sci Eng & Built Env

Department

School of Life & Env. Sciences

Campus

Geelong Waurn Ponds Campus

Qualifications

Doctor of Philosophy, University of Melbourne, 2003

Contact

m.mckenzie@deakin.edu.au
+61 3 522 73015

Biography

Dr Matthew McKenzie undertook his PhD with Dr Ian Trounce at St. Vincent's Hospital, Melbourne, where he developed novel mouse models of mitochondrial DNA (mtDNA) disease. He continued his mitochondrial research as a postdoctoral scientist in the laboratory of Professor Michael Duchen, University College London. There he investigated how mitochondrial membrane potential, calcium handling and ATP generation are disrupted in mitochondrial disorders using state-of-the-art confocal imaging techniques.

In 2004, Dr McKenzie returned to Australia to work with Professor Mike Ryan at La Trobe University, Melbourne. He received an NHMRC Peter Doherty Fellowship, and subsequently an NHMRC Career Development Fellowship, to study the biogenesis of mitochondrial complex I and how inherited defects can disrupt this process. In 2011, Dr McKenzie was recruited by the Hudson Institute of Medical Research as a Group Leader in the Centre for Genetic Diseases, where he established his own independent research program. 

Dr McKenzie was appointed as a Lecturer at Deakin University in 2018, with his current research focusing on the interactions between mitochondrial fatty acid beta-oxidation (FAO) and oxidative phosphorylation (OXPHOS) protein complexes and their importance in human disease pathology. He has obtained research funding from the NHMRC, the Australian Mitochondrial Disease Foundation and MEI Pharma, and was awared an ARC Future Fellowship in 2012 to support his research career.

Read more on Matthew's profile

Research interests

Mitochondria are the ‘powerhouses’ of eukaryotic cells, oxidizing sugars and fats to generate the energy molecule adenosine-5'-triphosphate (ATP). Defects in mitochondrial function can cause disease in both children and adults, which in many cases is fatal. Tissues with high energy demand, such as brain, heart and liver, are commonly affected, with few therapies available for treatment. Dr McKenzie’s research aims to define the pathological mechanisms which underlie these diseases, in particular how disruption of mitochondrial fatty acid beta-oxidation (FAO) and oxidative phosphorylation (OXPHOS) contribute to mitochondrial dysfunction.
Dr McKenzie’s group is defining the important physical interactions between FAO and OXPHOS protein complexes and determining how their disruption contributes to mitochondrial disease pathology. They are also investigating the role that various FAO proteins play in the biogenesis and assembly of the OXPHOS complexes. To do this, they use techniques such as Blue Native-PAGE, in vitro mitochondrial import and protein assembly assays. His group has also developed novel human models of FAO disease by reprogramming patient fibroblasts into induced pluripotent stem (iPS) cells and by CRISPR gene disruption in human ES cells. These stem cell lines can be differentiated into neurons, cardiomyocytes and hepatocytes to model mitochondrial disease pathology in a cell-type specific manner.

Teaching interests

Cell Biology, Human Disease, Metabolism

Units taught

SLE346 - Molecular Basis Of Disease

Knowledge areas

Mitochondrial biology; Mitochondrial Disease; Oxidative Phosphorylation; Fatty acid oxidation; Stem Cells

Conferences

2018: Scientific Program Committee, AussieMit 2018 Conference on Mitochondrial Biology and Disease, Melbourne

2013: Co-Chair, OzBio2013 Symposia, Perth

2012: Organizing Committee, AussieMit 2012 Conference on Mitochondrial Biology and Disease, Melbourne

2010: Co-Chair, OzBio2010 Colloquia, Melbourne

2008: Organizing Committee, the inaugural AussieMit Conference on Mitochondrial Biology and Disease, Melbourne

Professional activities

2017: NHMRC Grant Review Panel

2017: Project Grant Reviewer, University of Belgium and MRC DPFS Scheme, UK

2016:  Book Editor, Methods in Molecular Biology Series; ‘Mitochondrial DNA’, Springer

2015: Project Grant Reviewer, Boehringer Ingelheim Foundation, Germany

2013-2017: External Reviewer, ARC Discovery Projects and Fellowships

2012: Guest Editor: ‘Frontiers of Mitochondrial Research’, Biochim Biophys Acta - General Subjects

2011-2016: External Reviewer, NHMRC Project Grants

2010:  Project grant Reviewer, The Netherlands Organization for Scientific Research (NWO)

Awards

2012: Monash Research Accelerator Program

2008:  Junior Investigator Award, 33rd Lorne Conference on Protein Structure and Function

2002: Junior Investigator Award, St. Vincent's Hospital Research Week, Melbourne

Projects

Developing new stem cell models to investigate how defects in fatty acid oxidation contribute to human mitochondrial disease

Defining how microRNAs regulate mitochondrial metabolism

Developing novel therapeutic compounds for treating mitochondrial disease

Publications

Filter by

2019

Measurement of mitochondrial membrane potential with the fluorescent dye Tetramethylrhodamine Methyl Ester (TMRM)

Sarah Creed, Matthew McKenzie

(2019), Vol. 1928, pp. 69-76, Cancer metabolism, New York, N.Y., B1

chapter

Most clinical anti-EGFR antibodies do not neutralize both wtEGFR and EGFRvIII activation in glioma

Sameer Greenall, Mathew McKenzie, Ekatarina Seminova, Olan Dolezal, Lesley Pearce, John Bentley, Mani Kuchibhotla, Shengnan Chen, Kerrie McDonald, Harley Kornblum, Raelene Endersby, Timothy Adams, Terrance Johns

(2019), Vol. 21, pp. 1016-1027, Neuro-Oncology, Oxford, Eng., C1

journal
2018

Loss of the mitochondrial fatty acid ?-Oxidation protein medium-chain acyl-coenzyme A dehydrogenase disrupts oxidative phosphorylation protein complex stability and function

Sze Lim, Makiko Tajika, Masaru Shimura, Kirstyn Carey, David Stroud, Kei Murayama, Akira Ohtake, Matthew McKenzie

(2018), Vol. 8, pp. 1-17, Scientific Reports, London, Eng., C1-1

journal

Mitochondrial fatty acid oxidation disorders associated with short-chain Enoyl-CoA Hydratase (ECHS1) deficiency

Alice Sharpe, Matthew McKenzie

(2018), Vol. 7, pp. 1-13, Cells, Basel, Switzerland, C1-1

journal
2017

Monocytes and dendritic cells are the primary sources of interleukin 37 in human immune cells

Ina Rudloff, Steven Cho, Jason Lao, Devi Ngo, Matthew McKenzie, Claudia Nold-Petry, Marcel Nold

(2017), Vol. 101, pp. 901-911, Journal of leukocyte biology, Chichester, Eng., C1-1

journal

Simultaneous measurement of mitochondrial calcium and mitochondrial membrane potential in live cells by fluorescent microscopy

Matthew McKenzie, Sze Lim, Michael Duchen

(2017), pp. 1-6, Journal of visualized experiments, Cambridge, Ma., C1-1

journal

Mitochondrial DNA haplotypes induce differential patterns of DNA methylation that result in differential chromosomal gene expression patterns

William Lee, Xin Sun, Te-Sha Tsai, Jacqueline Johnson, Jodee Gould, Daniel Garama, Daniel Gough, Matthew McKenzie, Ian Trounce, Justin St John

(2017), Vol. 3, pp. 1-11, Cell Death Discovery, London, Eng., C1-1

journal
2016

Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability

Yilin Kang, Michael Baker, Michael Liem, Jade Louber, Matthew McKenzie, Ishara Atukorala, Ching-Seng Ang, Shivakumar Keerthikumar, Suresh Mathivanan, Diana Stojanovski

(2016), Vol. 5, pp. 1-23, eLife, Cambridge, Eng., C1-1

journal

Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly

Sze Lim, Jana Hroudov, Nicole Van Bergen, M Lopez Sanchez, Ian Trounce, Matthew McKenzie

(2016), Vol. 30, pp. 2236-2248, FASEB journal, Bethesda, Md., C1-1

journal

Dietary interventions designed to protect the perinatal brain from hypoxic-ischemic encephalopathy - Creatine prophylaxis and the need for multi-organ protection

Stacey Ellery, Hayley Dickinson, Matthew McKenzie, David Walker

(2016), Vol. 95, pp. 15-23, Neurochemistry international, Amsterdam, The Netherlands, C1-1

journal

Generation of xenomitochondrial embryonic stem cells for the production of live xenomitochondrial mice

Ian Trounce, Jessica Ackerley, Matthew McKenzie

(2016), pp. 163-173, Mitochondrial DNA : methods and protocols, Berlin, Germany, B1-1

chapter

Impaired cellular bioenergetics causes mitochondrial calcium handling defects in MT-ND5 mutant cybrids

Matthew McKenzie, Michael Duchen

(2016), Vol. 11, pp. 1-12, PLoS One, San Francisco, Calif., C1-1

journal

Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency

Gael Cagnone, Te-Sha Tsai, Yogeshwar Makanji, Pamela Matthews, Jodee Gould, Michael Bonkowski, Kirstin Elgass, Ashley Wong, Lindsay Wu, Matthew McKenzie, David Sinclair, Justin St John

(2016), Vol. 6, pp. 1-15, Scientific reports, London, Eng., C1-1

journal

Combined defects in oxidative phosphorylation and fatty acid ?-oxidation in mitochondrial disease

Abena Nsiah-Sefaa, Matthew McKenzie

(2016), Vol. 36, pp. 1-19, Bioscience reports, London, Eng., C1-1

journal

AarF domain containing kinase 3 (ADCK3) mutant cells display signs of oxidative stress, defects in mitochondrial homeostasis and lysosomal accumulation

Jason Cullen, Norazian Abdul Murad, Abrey Yeo, Matthew McKenzie, Micheal Ward, Kok Chong, Nicole Schieber, Robert Parton, Yi Lim, Ernst Wolvetang, Ghassan Maghzal, Roland Stocker, Martin Lavin

(2016), Vol. 11, pp. 1-28, PLoS One, San Francisco, Calif., C1-1

journal

Deletion of the complex I subunit NDUFS4 adversely modulates cellular differentiation.

Jacqueline Johnson, William Lee, Ann Frazier, Vijesh Vaghjiani, Adrienne Laskowski, Alexandra Rodriguez, Gael Cagnone, Matthew McKenzie, Stefan White, David Nisbet, David Thorburn, Justin St John

(2016), Vol. 25, pp. 239-250, Stem cells and development, New Rochelle, N.Y., C1-1

journal
2015

Analysis of Mitochondrial DNA in induced pluripotent and embryonic stem cells

William Lee, Richard Kelly, Ka Yeung, Gael Cagnone, Matthew McKenzie, Justin St John

(2015), Vol. 1330, pp. 219-252, Cell reprogramming : methods and protocols, Berlin, Germany, B1-1

chapter

Characterization of mitochondrial FOXRED1 in the assembly of respiratory chain complex I

Luke Formosa, Masakazu Mimaki, Ann Frazier, Matthew McKenzie, Tegan Stait, David Thorburn, David Stroud, Michael Ryan

(2015), Vol. 24, pp. 2952-2965, Human molecular genetics, Oxford, Eng., C1-1

journal

Anti-cancer analogues ME-143 and ME-344 exert toxicity by directly inhibiting mitochondrial NADH: Ubiquinone oxidoreductase (Complex I)

S Lim, K Carey, M McKenzie

(2015), Vol. 5, pp. 689-701, American Journal of Cancer Research, Madison, Wis., C1-1

journal
2014

A founder mutation in PET100 causes isolated complex IV deficiency in Lebanese individuals with Leigh syndrome

Sze Lim, Katherine Smith, David Stroud, Alison Compton, Elena Tucker, Ayan Dasvarma, Luke Gandolfo, Justine Marum, Matthew McKenzie, Heidi Peters, David Mowat, Peter Procopis, Bridget Wilcken, John Christodoulou, Garry Brown, Michael Ryan, Melanie Bahlo, David Thorburn

(2014), Vol. 94, pp. 209-222, American Journal of Human Genetics, Amsterdam, The Netherlands, C1-1

journal

The identification of mitochondrial DNA variants in glioblastoma multiforme

Ka Yeung, Adam Dickinson, Jacqueline Donoghue, Galina Polekhina, Stefan White, Dimitris Grammatopoulos, Matthew McKenzie, Terrance Johns, Justin St John

(2014), Vol. 2, pp. 1-21, Acta neuropathologica communications, London, England, C1-1

journal

Capture of somatic mtDNA point mutations with severe effects on oxidative phosphorylation in synaptosome cybrid clones from human brain

Matthew McKenzie, Maria Chiotis, Jana Hroudov, Maria Lopez Sanchez, Sze Lim, Mark Cook, Penny McKelvie, Richard Cotton, Michael Murphy, Justin St John, Ian Trounce

(2014), Vol. 35, pp. 1476-1484, Human mutation, London, Eng., C1-1

journal
2013

Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells

Richard Kelly, Andrew Rodda, Adam Dickinson, Arsalan Mahmud, Christian Nefzger, William Lee, John Forsythe, Jose Polo, Ian Trounce, Matthew McKenzie, David Nisbet, Justin St John

(2013), Vol. 31, pp. 703-716, Stem cells, Chichester, Eng., C1-1

journal

The effects of nuclear reprogramming on mitochondrial DNA replication

Richard Kelly, Huseyin Sumer, Matthew McKenzie, Joao Facucho-Oliveira, Ian Trounce, Paul Verma, Justin St John

(2013), Vol. 9, pp. 1-15, Stem cell reviews and reports, Cham, Switzerland, C1-1

journal

Mitochondrial DNA mutations and their effects on complex I biogenesis: implications for metabolic disease

M McKenzie

(2013), pp. 25-47, Mitochondrial DNA, mitochondria, disease and stem cells, New York, N.Y., B1-1

chapter

The regulation of mitochondrial DNA copy number in glioblastoma cells

A Dickinson, K Yeung, J Donoghue, M Baker, R Kelly, M McKenzie, T Johns, J St John

(2013), Vol. 20, pp. 1644-1653, Cell death & differentiation, London, Eng., C1-1

journal

Mitochondrial dysfunction in a novel form of autosomal recessive ataxia

Nor Murad, Jason Cullen, Matthew McKenzie, Michael Ryan, David Thorburn, Nuri Gueven, Junya Kobayashi, Geoff Birrell, Jian Yang, Thilo Drk, Olivier Becherel, Padraic Grattan-Smith, Martin Lavin

(2013), Vol. 13, pp. 235-245, Mitochondrion, Amsterdam, The Netherlands, C1-1

journal

Modulation of ceramide-induced cell death and superoxide production by mitochondrial DNA-encoded respiratory chain defects in Rattus xenocybrid mouse cells

Ian Trounce, Peter Crouch, Kirstyn Carey, Matthew McKenzie

(2013), Vol. 1827, pp. 817-825, Biochimica et biophysica acta (BBA) - bioenergetics, Amsterdam, The Netherlands, C1-1

journal
2012

Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A

Richard Kelly, Arsalan Mahmud, Matthew McKenzie, Ian Trounce, Justin St John

(2012), Vol. 40, pp. 10124-10138, Nucleic acids research, Oxford, Eng., C1-1

journal

Next-generation sequencing in molecular diagnosis: NUBPL mutations highlight the challenges of variant detection and interpretation

Elena Tucker, Masakazu Mimaki, Alison Compton, Matthew McKenzie, Michael Ryan, David Thorburn

(2012), Vol. 33, pp. 411-418, Human mutation, Chichester, Eng., C1-1

journal

Proteomic and metabolomic analyses of mitochondrial complex I-deficient mouse model generated by spontaneous B2 short interspersed nuclear element (SINE) insertion into NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) gene

Dillon Leong, Jasper Komen, Chelsee Hewitt, Estelle Arnaud, Matthew McKenzie, Belinda Phipson, Melanie Bahlo, Adrienne Laskowski, Sarah Kinkel, Gayle Davey, William Heath, Anne Voss, Ren Zahedi, James Pitt, Roman Chrast, Albert Sickmann, Michael Ryan, Gordon Smyth, David Thorburn, Hamish Scott

(2012), Vol. 287, pp. 20652-20663, Journal of biological chemistry, Rockville, Md., C1-1

journal

Understanding mitochondrial complex I assembly in health and disease

Masakazu Mimaki, Xiaonan Wang, Matthew McKenzie, David Thorburn, Michael Ryan

(2012), Vol. 1817, pp. 851-862, Biochimica et biophysica acta (BBA) - bioenergetics, Amsterdam, The Netherlands, C1-1

journal
2011

Mutations in the gene encoding C8orf38 block complex I assembly by inhibiting production of the mitochondria-encoded subunit ND1

Matthew McKenzie, Elena Tucker, Alison Compton, Michael Lazarou, Christa George, David Thorburn, Michael Ryan

(2011), Vol. 414, pp. 413-426, Journal of molecular biology, London, Eng., C1-1

journal

Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation

Elena Tucker, Steven Hershman, Caroline Khrer, Casey Belcher-Timme, Jinal Patel, Olga Goldberger, John Christodoulou, Jonathon Silberstein, Matthew McKenzie, Michael Ryan, Alison Compton, Jacob Jaffe, Steven Carr, Sarah Calvo, Uttam RajBhandary, David Thorburn, Vamsi Mootha

(2011), Vol. 14, pp. 428-434, Cell metabolism, Amsterdam, The Netherlands, C1-1

journal

Funded Projects at Deakin

No Funded Projects at Deakin found

Supervisions

No completed student supervisions to report