Prepare a submission
In accordance with section 2.1.8 of the Australian Code for the Care and Use of Animals for Scientific Purposes 8th Ed 2013 (the Code), institutions must ensure that all people involved in the care and use of animals understand their responsibilities and the requirements of the Code, are competent for the procedures they perform or are under the direct supervision of a person who is competent to perform the procedures and have access to appropriate education programs and resources.
Training is available for all Deakin University staff and students intending to and currently using animals for scientific purpose and described below.
Part 1: Regulatory issues in the care and use of animals for research and teaching at Deakin University
Part 1 training introduces individuals intending to use animals for scientific purposes to their responsibilities under the Victorian legislation. It is compulsory for individuals listed on an Animal Ethics application who intend to use live animals for scientific purposes.
Training is provided via CloudDeakin and the following steps must be followed in order to gain access:
- Go to Self Registration
- Select 'Office of Research Integrity'
- Click 'Register'
The quiz is accessible at the bottom of the 'Animal Ethics' page under 'Assessment'.
A score of 90% is required for successful completion and official notification will be provided once this has been achieved.
External investigators must be added to an Animal Ethics application before contacting the Animal Ethics Office to gain access to part 1 training.
Part 2: The care and use of laboratory rodents in research and teaching at Deakin University
Part 2 training is compulsory for investigators approved on an Animal Ethics application to learn the principles of laboratory rodent husbandry and gain practical skills essential for ensuring animal welfare is paramount.
The training is carried out over three separate occasions so that multiple opportunities to handle animals are provided prior to learning about specific procedures or until competency assessment is reached (additional sessions may be required). Individuals with prior experience must demonstrate competency and understanding of the Deakin University procedures on at least one occasion.
Register for training by submitting your details. You will be contacted by an Animal Research Integrity staff member to arrange suitable dates and times.
Part 3: Specific training provided by Principal Investigator or delegate
Part 3 training is provided by a Principal Investigator or delegate competent in performing a specific procedure(s), as identified in an Animal Ethics application and approved by the relevant Animal Ethics Committee (AEC).
Aseptic surgery training
Aseptic surgery training is compulsory for Deakin University staff and students approved on an Animal Ethics application to perform recovery surgery on animals.
The training is via CloudDeakin and consists of an online tutorial followed by a quiz that requires a score of 100% for successful completion.
The quiz is accessible at the bottom of the 'Animal Ethics' page under 'Assessment'.
The Animal Welfare Officer (AWO) conducts regular face-to-face training on the use of animals for scientific purposes at the Waurn Ponds campus and the Burwood campus. The training is designed to introduce new staff, Higher Degree Research (HDR) and Honours students to the Animal Ethics regulatory framework and to serve as a refresher for existing staff. An understanding of this framework is essential for navigating the Animal Ethics process and meeting legal responsibilities. Registration is via Eventbrite below.
Waurn Ponds Campus: Friday 14th February 2020 from 3pm - 4:30pm in room jb3.303
Waurn Ponds Campus: Tuesday 31st March 2020 from 11am - 12.30pm in room jb3.303
Waurn Ponds Campus: Tuesday 16th June 2020 from 11am - 12:30pm in room jb3.303
Burwood Campus: Wednesday 17th June 2020 from 11am - 12.30pm in room BC8.102
A Review Support Group is available to assist Deakin University staff and students in preparing an Animal Ethics submission. Please contact any or all of the following Research Integrity staff as required:
|Review Support Group||Name||Contact Details|
|Animal Welfare Officer||Dayna Johnsonfirstname.lastname@example.org|
|Laboratory Animal Supervisor||Adrian Cooperemail@example.com|
|Aviary and Wildlife Supervisor||Rod Collinsfirstname.lastname@example.org|
|Team Leader, Animal Research and Integrity||David Tayloremail@example.com|
|Senior Animal Ethics Coordinator||Erin Williamsfirstname.lastname@example.org|
- New application
- New application guidelines
- External project using animals at another Institution or in a country other than Australia
- Unexpected adverse event report
- Part 3 training
- 2019 annual report
- 2019 final report
- Permission to have an animal(s) on campus at Deakin University
- Use of a Deakin University SPPL room(s) by an external party
- Standard Operating Procedure (SOP) template
- ARRIVE Guidelines
- BAW classification of impact on animals
- NHMRC Best practice methodology in the use of animals for scientific purposes
- American Veterinary Medical Association (AVMA) Euthanasia Guidelines
Animal Research and Integrity
- National Health and Research Council (NHMRC) Animal Research Ethics
- Victorian Licensing Authority - DEDJTR
- Canadian Council On Animal Care
- European Centre for the Validation of Alternative Methods (ECVAM) publications
- UK National National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
- Lab Animal Magazine
- American Association For Laboratory Animal Science (AALAS)
- Australian and New Zealand Council For The Care Of Animals in Research and Teaching (ANZCCART)
- New South Wales Agriculture
- Animal Welfare Information Centre (AWIC)
- Laboratory Animals - Online Reprints
- Universities Federation For Animal Welfare (UFAW)
- The Norwegian Reference Centre For Laboratory Animal Science & Alternatives
Standard Operating Procedures (SOPs) are available to Deakin University staff and students with the purpose to provide a step-by-step description of a specific procedure and provide further information to that offered in an Animal Ethics application. They are also intended to improve consistency where applicable, attempt to reduce miscommunication and failure to comply with relevant legislation.
SOPs do not replace information being provided in an Animal Ethics application but rather can be used as an accompaniment to better describe a specific task or operation. Furthermore, SOPs will not be assessed as stand-alone submissions to an Animal Ethics Committee, as they must relate directly to an application.
If you wish to create a new SOP for assessment with an Animal Ethics application, please use the Deakin University New Standard Operating Procedure (SOP) form.
Husbandry Standard Operating Procedures (SOPs)
- SOP02-2013 Cane toad pain and distress monitoring (DOCX, 52.8 KB)
- SOP02-2015 Guppy husbandry (DOCX, 55.6KB)
- SOP02-2017 Rodent identification and ear punch for genotyping (DOCX, 64.7 KB)
- SOP03-2014 Husbandry of fish and yabbies (DOCX, 58.4 KB)
- SOP03-2017 Transport of sub adult and adult aquatic GM organisms between certified facilities (DOCX, 55.1 KB)
- SOP04-2013 Husbandry of zebra finch breeding colony (DOCX, 54.4 KB)
- SOP04-2017 Transport of larval aquatic GM organisms between certified facilities (DOCX, 55.2 KB)
- SOP06-2014 Procedure for sick or injured research birds (DOCX, 52.6 KB)
- SOP06-2015 Zebra fish husbandry (DOCX, 57.1 KB)
- SOP07-2014 Cane toad husbandry (DOCX, 970.0 KB)
- SOP07-2017 Procedure for rodents found sick, injured or dead in an Animal Facility (DOCX, 54.0 KB)
- SOP08-2014 Rabbit husbandry (DOCX, 54.9 KB)
- SOP09-2014 Rabbit environmental enrichment (DOCX, 56.3 KB)
- SOP09-2015 Rodent husbandry (DOCX, 59.4 KB)
- SOP10-2014 Zebra finch husbandry (DOCX, 54.6KB)
- SOP10-2015 Environmental enrichment for rodents (DOCX, 58.2 KB)
- SOP11-2015 Husbandry of tadpoles (DOC, 101.0 KB)
- SOP42-2011 DAF fish husbandry and standard procedures (DOCX, 106.9KB)
- SOP60-2011 Crimson rosella housing in outdoor aviaries (DOCX, 55.5 KB)
- SOP92-2010 Aviary enrichment for captive birds (DOCX, 53.2 KB)
Scientific Standard Operating Procedures (SOPs)
- SOP01-2012 Breast cancer orthopic xenograft model in NODSCID mice (DOCX, 118.9 KB)
- SOP01-2013 Cannulation for hyperinsulinaemic euglycaemic clamp (DOCX, 55.8 KB)
- SOP01-2014 Rosella blood sampling (DOCX, 132.7 KB)
- SOP01-2015 Fyke netting and bait trapping (DOCX, 456.9 KB)
- SOP01-2016 Remote camera surveys of fauna (DOCX, 58.0 KB)
- SOP01-2017 Emergency euthanasia for fur seals KCI (DOCX, 334.9 KB)
- SOP01-2018 Muscle function testing (DOCX, 1.6MB)
- SOP02-2014 Subcutaneous tumour cell injections in mice (DOCX, 245.9 KB)
- SOP02-2016 Sampling southern rock lobster with commercial fishing methods (DOCX, 2.4 MB)
- SOP02-2018 Visible implant alpha tags (DOCX, 2.1MB)
- SOP03-2012 Recording colour patterns of live fish (DOCX, 54.8 KB)
- SOP03-2013 Cane toad humane killing techniques (DOCX, 52.8 KB)
- SOP03-2015 Dosing of nestling zebra finch with corticosterone (DOCX, 53.5 KB)
- SOP03-2016 Oral gavage of rodents (DOCX, 57.2 KB)
- SOP04-2014 Recording resting metabolic rate of fish and yabbies (DOCX, 56.2 KB)
- SOP04-2016 Rosella capture in nestbox traps (DOCX, 115.8 KB)
- SOP05-2012 DBS stereotaxic surgery (DOCX, 55.1 KB)
- SOP05-2013 Subcutaneous injection (DOCX, 63.7 KB)
- SOP05-2014 Activity and boldness assays for fish (DOCX, 55.5 KB)
- SOP05-2016 Cloacal swab sampling of birds (DOCX, 53.8 KB)
- SOP06-2012 Anaesthetising mice prior for imaging with the IVIS Lumina II Bioluminescence Camera (DOCX, 68.2KB)
- SOP06-2013 Intravenous injection
- SOP06-2016 PHA immune challenge in birds (DOCX, 56.0 KB)
- SOP06-2017 Koala Trapping – Capture Handling and Release (DOCX, 1.6 MB)
- SOP07-2013 Tail blood smears (DOCX, 52.9 KB)
- SOP07-2015 Visible implant elastomer tagging in zebrafish (DOCX, 58.9 KB)
- SOP07-2016 Amputation of an anuran toe for use in skeletochronology (DOCX, 74.0 KB)
- SOP08-2012 Capture of wild birds using net guns (DOCX, 55.0 KB)
- SOP08-2015 In Vitro fertilization of zebrafish embryo (live) (DOCX, 60.5 KB)
- SOP08-2016 Humane killing of zebrafish using Benzocaine (DOCX, 55.3 KB)
- SOP08-2017 PIT tagging (DOCX, 74.6KB)
- SOP09-2012 In vitro testing of skeletal muscle contractile properties (DOCX, 53.3 KB)
- SOP09-2016 Brain collection from zebra finch embryos (DOCX, 52.9 KB)
- SOP09-2017 Fyke netting and bait trapping (aquatic environments) (DOCX, 921.0KB)
- SOP10-2012 Saphenous vein bleeding (DOCX, 53.2 KB)
- SOP10-2016 Blood sampling birds (DOCX, 105.4 KB)
- SOP11-2014 Blood collection techniques (DOCX, 58.7 KB)
- SOP12-2009 Perfusion (DOCX, 53.0 KB)
- SOP12-2012 Capturing colony nesting seabirds using a noose-pole (DOCX, 53.4 KB)
- SOP12-2014 Injection techniques and routes of administration (DOCX, 60.1 KB)
- SOP12-2015 Tadpole metabolic rate (DOC, 95.5 KB)
- SOP13-2014 Isoflurane anaesthesia (PDF, 515.3 KB)
- SOP13-2015 Activity and boldness assays for tadpoles (DOC, 94.5 KB)
- SOP14-2012 Hook and line capture of fish (DOCX, 54.1 KB)
- SOP14-2014 Protocol for crab capture-baited traps (DOC, 74.0 KB)
- SOP14-2015 Catching and humane killing of tadpoles (DOC, 96.5 KB)
- SOP15-2009 Feather sampling of wild birds (DOCX, 57.2 KB)
- SOP15-2011 Attachment of external data loggers to fur seals (DOCX, 54.4 KB)
- SOP15-2014 Fish euthanasia via physical methods (DOC, 73.5 KB)
- SOP16-2009 Hand capture of wild birds (DOCX, 56.1 KB)
- SOP16-2011 Blood sampling of fur seals (DOCX, 53.9 KB)
- SOP16-2014 Protocol for general use of small trawl nets (DOC, 75.5 KB)
- SOP17-2009 Capture of wild birds using mist nets (DOCX, 58.6 KB)
- SOP17-2011 Capture and restraint of fur seals (DOCX, 55.4 KB)
- SOP18-2009 Measuring bird eggs (DOCX, 59.1 KB)
- SOP18-2011 Milk sampling of fur seals (DOCX, 53.5 KB)
- SOP19-2009 Buccal swab samplings of wild birds (DOCX, 55.8 KB)
- SOP19-2011 Tooth extraction in fur seals and sea lions (DOCX, 53.8 KB)
- SOP20-2009 Shorebird capture techniques (DOCX, 58.2 KB)
- SOP21-2009 Active searching for reptiles and amphibians (DOCX, 54.2 KB)
- SOP22-2009 Pitfall trapping of reptiles, amphibians and small mammals (DOCX, 57.3 KB)
- SOP23-2009 Mammal trapping using Elliot and cage traps (DOCX, 60.5 KB)
- SOP24-2009 Spotlighting surveys of nocturnal fauna (DOCX, 55.8KB)
- SOP39-2011 Rosella uropygial secretion sampling (DOCX, 58.0 KB)
- SOP43-2011 Catching of guppies followed by humane killing with MS-222 (DOCX, 54.5 KB)
- SOP51-2010 Commercial pigeon housing and husbandry (DOCX, 53.9 KB)
- SOP59-2011 Rosella behavioural choice tests (DOCX, 206.5 KB)
- SOP61-2011 Banding and colour banding of small to medium passerines and collecting body measurements (DOCX, 806.7 KB)
- SOP62-2010 Deployment of external data-logging devices on seabirds (DOCX, 190.6 KB)
- SOP64-2011 Zebra fish tail clip (DOCX, 52.7 KB)
- SOP66-2010 Subcutaneous copper salt injections of mottled brindled mice (DOCX, 52.8 KB)
- SOP69-2010 Surgical implantation of tumour cells into rat brain (DOCX, 54.4 KB)
- SOP70-2010 Tail Vein Injection (DOCX, 54.2 KB)
- SOP89-2010 Forced swim test (DOCX, 52.7 KB)
- SOP90-2010 Open field test (DOCX, 52.4 KB)
- SOP96-2011 Rodent humane killing techniques (DOCX, 65.1 KB)
- SOP97-2011 Avian humane killing techniques (DOCX, 64.0 KB)
- SOP111-2010 Infection of donor mouse or rat with malaria parasites (DOCX, 53.9 KB)
- SOP112-2010 Cerebral malaria model (DOCX, 54.1 KB)
- SOP115-2010 Tumour subcutaneous xenograft model in nude mice (DOCX, 245.4 KB)
- SOP116-2010 Koala, capture, handling and release (DOCX, 57.3 KB)
It is requirement under Section 1.21 of the Australian Code for the Care and Use of Animals for Scientific Purposes 8th Ed 2013 (the Code) that the number of animals used in a project must be the minimum necessary to achieve the proposed aim(s) and to satisfy good statistical design. Well designed and correctly analysed experiments can lead to a reduction in animal use whilst increasing the scientific validity of the results.
A well designed experiment should be:
When two or more treatment groups are compared, the animals in the groups should be in identical environments and be similar in every way apart from the applied treatments. Bias can be minimised by:
- Randomisation - randomly allocating animals to the treatment groups (a physical process is needed e.g. tossing a coin, picking a number)
- Ensuring that all subsequent treatments (including housing) are applied in a random order
- Blinding - Ensuring that researchers analysing experimental outcomes are unaware of the treatment received (blinded) until the final statistical analysis
Adequately powered (i.e. use sufficient animals)
Powerful experiments are ones that have the maximum chance of detecting a true treatment effect. Power is achieved by:
- Using appropriate numbers of animals (sample size)
- Controlling inter-subject variation (e.g. using randomisation)
Sample size should be determined using a formal method such as power analysis or using the resource equation method. Although power is increased by increasing sample size, an unnecessarily large experiment will waste animals and scientific resources.
Variation is controlled by randomly allocating animals of similar genotypes, of a similar weight and age, which have had a similar environment throughout their lives. Variation due to circadian rhythms or fluctuations in the environment can often be reduced by appropriate experimental design, by using a randomised block or Latin square experiments.
Measurement error should be minimised by careful technique and good instrumentation, and blinding the researcher to treatment allocation.
A power analysis comparing two groups, for example, requires the following information:
- The type of statistical test to be used (e.g. a t-test or a chi-squared test to compare two proportions)
- The significance level to be used (often a 5% level)
- The required statistical power (usually 80-90%)
- The sidedness of the test (a 2-sided test is usual)
- The effect size of biological interest (i.e. how much of a difference in biological or clinical effect it is necessary to detect)
- An estimate of the standard deviation (when comparing means) this must come from a previous experiment
Have a wide range of applicability
It is often useful to find out whether similar results are obtained in males and females, in different strains, or as a result of different diets or environments.
Be simple and efficient
Experiments should not be so complicated that mistakes are made in their execution, or the statistical analysis becomes unduly complicated. Small pilot studies should be used before starting a major experiment to ensure that the experiment is logistically efficient and to give some preliminary indication of likely results.
Indicate the range of certainty
Each experiment should be statistically analysed so that the results can be used in planning future experiments. An appropriate statistical analysis should indicate the range of uncertainty in the results, or the measure of variation, usually indicated by significance levels or confidence intervals.
A biostatistician from your Faculty must be conducted prior to submission of a new Animal Ethics Application and further information on statistical design can be found at The Experimental Design Assistant.
|AECW-B meeting |
|AECL-G meeting |
|1/20||20 January 2020||5 February 2020||12 February 2020|
|2/20||17 February 2020||4 March 2020||18 March 2020|
|3/20||30 March 2020||15 April 2020||29 April 2020|
|4/20||11 May 2020||27 May 2020||10 June 2020|
|5/20||22 June 2020||8 July 2020||22 July 2020|
|6/20||3 August 2020||19 August 2020||2 September 2020|
|7/20||21 September 2020||7 October 2020||21 October 2020|
|8/20||9 November 2020||25 November 2020||2 December 2020|