ITRI

Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)

BioDeakin staff profiles

Dr Munish Puri

Dr Munish Puri

Position: Research Fellow (BioProcessing)
Phone: +61 3 522 72325
Email: munish.puri@deakin.edu.au
Campus: G


 

Research interests:

Dr Puri has rich experience in protein biotechnology which includes protein chemistry, enzyme technology and bio-processing. The major goals of my laboratory are to produce and purify novel therapeutic proteins/ enzymes from variety of sources (microbes, animal and plant cell) and to improve their functional efficiency, specific activity for carrying out transformations of flavonoid/ pharmaceutical intermediates/animal cell product with health benefits. We follow molecular biology, bio-processing and protein engineering approaches for enhancing the efficiency of various enzymes (few eg: rhamnosidases, aspartase, inulinase, proteases, etc)/ proteins (ribosome inactivating proteins, macrophage proteins etc) with therapeutic benefits. We are also working on the biophysical characterization and crystallization of novel proteins to evaluate rigidity of 3D structure which is of paramount importance in immobilization and also for assigning new function to proteins.

Qualifications:

  • PhD Biotechnology, Punjabi University, India
  • MSc Biotechnology, Punjabi University, India

Relevant employment history:

2009 - present: Research Fellow Bio-processing, Deakin University, Australia
2008: Visiting Professor ITRI (LIMBR), Deakin University, Australia
2004 - 2005: Research Scientist IMB, University of Queensland (UQ), Australia
2003 - 2008: *Associate Professor Biotechnology, Punjabi University, India (*on leave)
2002 - 2003: Commonwealth Fellow, University of Oxford, UK and Visiting Scientist,
1998 - 2001: Senior Lecturer, Punjabi University, India
1997 - 1998: National Associate, Institute of Microbial Technology (IMTECH), India

A list of the ten most significant relevant publications for the last five years:

  1. Puri M, Sharma D, Barrow CJ. 2011. Enzyme assisted extraction of bioactives from plants. Trends in Biotechnology (doi:10.1016/j.tibtech.2011.06.014, Impact factor 9.64)
  2. Puri M, Kaur A, Barrow C J, Singh R S. 2011. Citrus peel enhances production of a novel naringinase in a stirred tank reactor by Staphylococcus xylosus MAK2. Applied Microbiology and Biotechnology 89 (3), 715-722 (Impact factor 3.284).
  3. Kapardar R, Ranjan R, Grover A, Puri M, Sharma R. 2010. Identification and characterization of genes conferring salt tolerance to E.coli from pond water metagenome. Bioresource Technology 101(11) 3917-3924 (Impact factor 4.4)
  4. Kaur A, Singh RS, Schwarz WH. Puri M. 2010. Hydrolysis of citrus peel naringin by recombinant-L-rhamnosidase from Clostridium stercorarium. Journal of Chemical Technology Biotechnology 85, 1419-1422 (Impact factor 2.045)
  5. Puri M, Kaur A, Singh RS, Schwarz WH, Kaur A. 2010. One step purification and immobilization of His-tagged rhamnosidase for narigin hydrolysis. Process Biochemistry 45 (4), 445-451 (Impact factor 2.648)
  6. Puri M, Kaur I, Kanwar RK, Gupta, RC, Chauhan AK, Kanwar JR. 2009. Ribosome inactivating proteins for antiviral therapy. Current Molecular Medicine 9, 1080-94. (Impact factor 5.212)
  7. Drinkwater N, Gee CL, Puri M, Criscione KR, McLeish MJ, Grunewald GL, Martin JL. 2009. Molecular recognition of physiological substrate noradrenaline by the adrenaline synthesising enzyme PNMT and factors influencing its methyltransferase activity. Biochemical Journal 422 (3), 463-71. (Impact factor 5.016)
  8. Singh RS, Dhaliwal R, Puri M. 2008. Development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin. Journal of Industrial Microbiology and Biotechnology 35(7):777-82. (Impact factor IF 2.41)
  9. Singh RS, Sooch B, Puri M. 2007. Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1. Bioresource Technology 98 (13): 2518-2525. (Impact factor IF 4.4)
  10. Welford RWD, Kirkpatrick JM, McNeill L, Puri M, Oldham NJ. and Schofield C J. 2005. Incorporation of oxygen into the succinate co-product of iron (II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans. FEBS Letters 579:5170-5174. (Impact factor 3.6)
  11. Puri M, Banerjee UC. 2000. Production, purification and characterization of the debittering enzyme naringinase. Biotechnology Advances 18, 207- 217. (Impact factor 9.03)

University recorded publications

Researcher output profile for Dr Munish Puri

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16th June 2011