A/Prof. Luis Afonso

STAFF PROFILE

Position

Director, International Research Training

Faculty

Deakin Research

Department

DR - Int'l Partnerships

Campus

Geelong Waurn Ponds Campus

Qualifications

Doctor of Philosophy, Univ. of British Columbia, 1997

Contact

luis.afonso@deakin.edu.au
+61 3 556 33461

Biography summary

Dr. Luis Afonso is a fish physiologist with an interest in fish health (stress) and reproduction. Luis completed an MSc degree at the Federal University of Rio Grande do Sul (Brazil – 1992) on sex reversal in fish, and a PhD at the University of British Columbia (Canada - 1997) on reproductive endocrinology of Pacific salmon.
After his PhD, Luis took on an associate professor position at the Federal University of Rio Grande do Sul (1997-2000), and he worked closely with the aquaculture industry on research and development projects aimed at producing monosex population of Nile tilapia.
Luis returned to Canada in 2000 to take on a Visiting Scientist position with the Department of Fisheries and Oceans Canada to work on the effects of environmental pollutants during sexual development in fish. In 2001 he joined the National Research Council of Canada, Institute for Marine Biosciences (Halifax-Canada) where he developed a research program on stress in fish. From 2009-2012, Luis took up a Senior Research position with the BC Centre for Aquatic Health Sciences (Campbell River-Canada) to work on fish health.
For the past 15 years, Luis has been working closely not only with his colleagues at academia but also with the private sector, as he thinks that an interdisciplinary team based approach is vital for understanding, maintaining, and advancing  our knowledge in life sciences.

Career highlights

Senior Lecturer in Marine Sciences, Deakin University, Australia, 2012-present
Senior Research Scientist, BC Centre for Aquatic Health Sciences, Canada, 2009-2012
Research and Development, Genome Atlantic, Canada, 2007-2009.
Research Officer, National Research Council Canada (NRC), Canada, 2001-2007
Visiting Scientist in Canadian Government Laboratory, Fisheries and Oceans Canada, Canada, 2000-2001
Associate Professor, Federal University of Rio Grande do Sul,  Brazil, 1997-2000

Adjunct Appointments
• Adjunct Professor (2009-2012) Vancouver Island University, Nanaimo, BC, Canada.
• Adjunct Professor (2004-2007) Memorial University of Newfoundland, Ocean   Science Centre, St. John’s, NL, Canada.
• Adjunct Professor (2000-2004) Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

Research interests

- Fish and invertebrate stress, health, diseases, and welfare
- Stress response to environmental and aquaculture-related stressors: thermal stress, hypoxia, environmental toxicants, crowding, transportation and harvesting
- Individual variation in stress response
- Heath-shock proteins, enzymes involved in the synthesis of corticosteroids (StAR, P450scc, 3β-HSD) cortisol bound and free, glucose, lactate, and glycogen, expression of immune-related genes, sex steroid hormones
- Genomics, proteomics and metabolomics
- Fish reproductive endocrinology, sex differentiation, and sex reversal
- Marine and freshwater finfish and shellfish, aquaculture
- Recreational fishing: stress and welfare
- Stress and nutrition
- Development of biomarkers of stress and health
- Development of diagnostic techniques using genomic approaches

Teaching interests

Animal physiology, cell biology, endocrionology, stress physiology, reproduction, aquaculture

Units taught

SLE111 - Cells and genes

SLE254 - Genetics (practicals)

SLE315 - Comparative animal physiology (selected topics)

SLE329 - Aquatic animal  health and reproduction

Knowledge areas

Fish physiology, endocrinology,  reproduction, stress, health, aquaculture, molecular biology, sex reversal, sex control, fish welfare

Professional activities

Reviewer for academic journals and funding agencies
• Aquaculture, Aquaculture Reviews, Comparative Biochemistry and Physiology, Fish Physiology and Biochemistry, Fish and Shellfish Immunology, General and Comparative Endocrinology, Journal of Fish Biology, Molecular and Cellular Endocrinology, North American Journal of Aquaculture, Journal of Aquaculture Research and Development.
• Reviewer for granting agencies in Canada (NSERC) and Brazil (CNPq, CAPES, FAPERGS).
• USDA, Cooperative State Research, Education, and Extension Service Small Business Innovation Research.

Awards

Awards and Academic Achievements
• British Columbia Aquaculture and Environment Innovation Award – (British Columbia Innovation Council (April 2009 – January 2012), Research Chair in Aquaculture and Environment.
• Natural Sciences and Engineering Research Council of Canada - NSERC (September 2000 - August 2001),Visiting Scientist in Canadian Government Laboratory.
• Federal University of Rio Grande do Sul, Association of Graduate Studies in Animal Science (September 2000), Award for Excellence in Graduate Studies Administration.
• Brazilian Society of Animal Sciences (1998-1999), General Secretary of the XXXVI Annual Meeting of the Brazilian Society of Animal Science - Porto Alegre, Brazil.
• Brazilian National Research Council - CNPq (August 1998 - August 2000), Award for Excellence in Research.
• Brazilian National Research Council - CNPq (1994-1997), Award for PhD degree.
• Brazilian Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior - CAPES (1990-1992), Award for MSc degree.

Publications

Filter by

2016

Effects of commercial diets and temperature on the growth performance and stress response of hapuku (Polyprion oxygeneios)

J Tromp, P Jones, J Symonds, S Walker, A Pope, S Pether, L Afonso

(2016), Vol. 452, pp. 128-133, Aquaculture, Amsterdam, The Netherlands, C1

journal

Long-term epidemiological survey of Kudoa thyrsites (Myxozoa) in Atlantic salmon (Salmo salar L.) from commercial aquaculture farms

W Marshall, A Sitjà-Bobadilla, H Brown, T MacWilliam, Z Richmond, H Lamson, D Morrison, L Afonso

(2016), Vol. 39, pp. 929-946, Journal of fish diseases, Chichester, Eng., C1

journal
2015

Effects of thermal stressors on growth-related gene expressions in cutured fish

T Nakano, M Kameda, T Yamaguchi, M Sato, L Afonso, G Iwama, R Devlin

(2015), pp. 147-157, Marine productivity: perturbations and resilience of socio-ecosystems : proceedings of the 15th French-Japanese Oceanography Symposium, Berlin, Germany, B1

chapter
2013

Transcriptome analysis of neoplastic hemocytes in soft-shell clams Mya arenaria : focus on cell cycle molecular mechanism

A Siah, P McKenna, F Berthe, L Afonso, J Danger

(2013), Vol. 3, pp. 95-103, Results in immunology, Amsterdam, The Netherlands, C1

journal

Acute physiological stress down-regulates mRNA expressions of growth-related genes in coho salmon

T Nakano, L Afonso, B Beckman, G Iwama, R Devlin

(2013), Vol. 8, pp. 1-7, PLoS ONE, San Francisco, Calif., C1

journal
2012

Inter-individual and -family differences in the cortisol responsiveness of Atlantic cod (Gadus morhua)

T Hori, A Gamperl, C Hastings, G Voort, J Robinson, S Johnson, L Afonso

(2012), Vol. 324-325, pp. 165-173, Aquaculture, Amsterdam, The Netherlands, C1-1

journal

The mRNA expression of cortisol axis related genes differs in Atlantic cod (Gadus morhua) categorized as high or low responders

T Hori, M Rise, S Johnson, L Afonso, A Gamperl

(2012), Vol. 175, pp. 311-320, General and comparative endocrinology, Maryland Heights, Mo., C1-1

journal

Use of ultraviolet C (UVC) radiation to inactivate infectious hematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV) in fish processing plant effluent

L Afonso, Z Richmond, A Eaves, J Richard, L Hawley, K Garver

(2012), Vol. 3, pp. 1-5, Journal of aquaculture research and development, Los Angeles, Calif., C1-1

journal
2010

Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques

T Hori, A Gamperl, L Afonso, S Johnson, S Hubert, J Kimball, S Bowman, M Rise

(2010), Vol. 11, pp. 1-22, BMC genomics, London, England, C1-1

journal

Is permethylation strategy always applicable to protein N-glycosylation study? A case study on the O-acetylation of sialic acid in fish serum glycans

X Liu, L Afonso

(2010), pp. 259-268, Functional glycomics: methods and protocols, New York, N. Y., B1-1

chapter
2009

1H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress

T Karakach, E Huenupi, E Soo, J Walter, L Afonso

(2009), Vol. 5, pp. 123-137, Metabolomics, New York, N. Y., C1-1

journal

Hemoglobin genotype has minimal influence on the physiological response of juvenile atlantic cod (Gadus morhua) to environmental challenges

A Gamperl, C Busby, T Hori, L Afonso, J Hall

(2009), Vol. 82, pp. 483-494, Physiological and biochemical zoology, Chicago, Ill., C1-1

journal
2008

Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus) subjected to a sudden cold shock

L Inoue, G Moraes, G Iwama, L Afonso

(2008), Vol. 38, pp. 603-609, Acta amazonica, Manaus, Brasil, C1-1

journal

Impairment of the stress response in matrinxã juveniles (Brycon amazonicus) exposed to low concentrations of phenol

T Hori, I Avilez, G Iwama, S Johnson, G Moraes, L Afonso

(2008), Vol. 147, pp. 416-423, Comparative biochemistry and physiology part C, Philadelphia, Pa., C1-1

journal

O-acetylation of sialic acids in N-glycans of Atlantic salmon (Salmo salar) serum is altered by handling stress

X Liu, L Afonso, E Altman, S Johnson, L Brown, J Li

(2008), Vol. 8, pp. 2849-2857, Proteomics, Weinheim, Germany, C1-1

journal

The immune and stress responses of Atlantic cod to long-term increases in water temperature

J Perez-Casanova, M Rise, B Dixon, L Afonso, J Hall, S Johnson, A Gamperl

(2008), Vol. 24, pp. 600-609, Fish and shellfish immunology, London, England, C1-1

journal

Cortisol response and immune-related effects of Atlantic salmon (Salmo salar Linnaeus) subjected to short- and long-term stress

M Fast, S Hosoya, S Johnson, L Afonso

(2008), Vol. 24, pp. 194-204, Fish and shellfish immunology, England, London, C1-1

journal

The stress and metabolic responses of juvenile Atlantic cod Gadus morhua L. to an acute thermal challenge

J Pérez-Casanova, L Afonso, S Johnson, S Currie, A Gamperl

(2008), Vol. 72, pp. 899-916, Journal of fish biology, Oxford, England, C1-1

journal

Lack of glucose and hsp70 responses in haddock Melanogrammus aeglefinus (L.) subjected to handling and heat shock

L Afonso, S Hosoya, J Osborne, A Gamperl, S Johnson

(2008), Vol. 72, pp. 157-167, Journal of fish biology, Oxford, England, C1-1

journal
2007

Effects of moderately oxidized dietary lipid and the role of vitamin E on the stress response in Atlantic halibut (Hippoglossus hippoglossus L.)

D Martins, L Afonso, S Hosoya, L Lewis-McCrea, L Valente, S Lall

(2007), Vol. 272, pp. 573-580, Aquaculture, Amsterdam, The Netherlands, C1-1

journal

Changes in free and total plasma cortisol levels in juvenile haddock (Melanogrammus aeglefinus) exposed to long-term handling stress

S Hosoya, S Johnson, G Iwama, A Gamperl, L Afonso

(2007), Vol. 146, pp. 78-86, Comparative biochemistry and physiology part A, Philadelphia, Pa., C1-1

journal
2006

Triploid and diploid rainbow trout do not differ in their stress response to transportation

R Leggatt, K Scheer, L Afonso, G Iwama

(2006), Vol. 68, pp. 1-8, North American journal of aquaculture, Bethesda, Md., C1-1

journal

Stress in fish

G Iwama, L Afonso, M Vijayan

(2006), pp. 320-342, Physiology of fishes, Boca Raton, Flo., B1-1

chapter
2005

Effects of clove oil on the stress response of matrinxã (Brycon cephalus) subjected to transport

L Inoue, L Afonso, G Iwama, G Moraes

(2005), Vol. 35, pp. 289-295, Acta amazonica, Manaus, Brazil, C1-1

journal
2004

Stress response of juvenile rainbow trout (Oncorhynchus mykiss)to chemical cues released from stressed conspecifics

D Toa, L Afonso, G Iwama

(2004), Vol. 30, pp. 103-108, Fish physiology and biochemistry, Amsterdam, The Netherlands, C1-1

journal

Are hsps suitable for indicating stressed states in fish?

G Iwama, L Afonso, A Todgham, P Ackerman, K Nakano

(2004), Vol. 207, pp. 15-19, Journal of evolutionary biology, Oxford, England, C1-1

journal
2003

Sex reversal in Nile tilapia (Oreochromis niloticus Linnaeus) by androgen immersion

G Wassermann, L Afonso

(2003), Vol. 34, pp. 65-71, Aquaculture research, Oxford, England, C1-1

journal

Sex-related differences in the organismal and cellular stress response in juvenile salmon exposed to treated bleached kraft mill effluent

L Afonso, N Basu, K Nakano, R Devlin, G Iwama

(2003), Vol. 29, pp. 173-179, Fish physiology and biochemistry, Amsterdam, The Netherlands, C1-1

journal
2002

Y-chromosomal DNA markers for discrimination of chemical substance and effluent effects on sexual differentiation in Salmon

L Afonso, J Smith, M Ikonomou, R Devlin

(2002), Vol. 110, pp. 881-887, Environmental health perspectives, Research Triangle Park, N.C., C1-1

journal

Tecnica simples de marcacao externa de reprodutores de tilapia nilotica (Oreochromis niloticus)

E Leboute, L Afonso, M Rotta

(2002), Vol. 32, pp. 147-149, Ciência rural, Santa Maria, Brasil, C1-1

journal

Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry

G Wassermann, L Afonso

(2002), Vol. 32, pp. 133-139, Ciência rural, Santa Maria, Brasil, C1-1

journal
2001

Sex reversal in Nile tilapia (Oreochromis niloticus) using a nonsteroidal aromatase inhibitor

L Afonso, G Wassermann, R de Oliveira

(2001), Vol. 290, pp. 177-181, Journal of experimental zoology : part A - comparative experimental biology, Hoboken, N. J., C1-1

journal

Tecnica simples para identificacao de femeas de tilapia nilotica (Oreochromis niloticus) realizando incubacao oral dos ovos

M Rotta, L Afonso

(2001), Vol. 31, pp. 521-522, Ciência rural, Santa Maria, Brasil, C1-1

journal
2000

Effects of the aromatase inhibitor Fadrozole on reproductive steroids and spermiation in male coho salmon (Oncorhynchus kisutch) during sexual maturation

L Afonso, G Iwama, J Smith, E Donaldson

(2000), Vol. 188, pp. 175-187, Aquaculture, Amsterdam, The Netherlands, C1-1

journal
1999

Effects of the aromatase inhibitor Fadrozole on plasma sex steroid secretion and oocyte maturation in female coho salmon (Oncorhynchus kisutch) during vitellogenesis

L Afonso, G Iwama, J Smith, E Donaldson

(1999), Vol. 20, pp. 231-241, Fish physiology and biochemistry, Amsterdam, The Netherlands, C1-1

journal

Effects of the aromatase inhibitor Fadrozole on plasma sex steroid secretion and ovulation rate in female coho salmon, oncorhynchus kisutch, close to final maturation

L Afonso, G Iwama, J Smith, E Donaldson

(1999), Vol. 113, pp. 221-229, General and comparative endocrinology, Maryland Heights, Mo., C1-1

journal
1997

The effect of the aromatase inhibitor fadrozole and two polynuclear aromatic hydrocarbons on sex steroid secretion by ovarian follicles of coho salmon

L Afonso, P Campbell, G Iwama, R Devlin, E Donaldson

(1997), Vol. 106, pp. 169-174, General and comparative endocrinology, Maryland Heights, Mo., C1-1

journal
1993

Method for artificial incubation of Nile tilapia (Oreochromis niloticus) eggs

L Afonso, E Leboute, S Souza

(1993), Vol. 22, pp. 502-505, Revista Brasileira de zootecnia, Minas Gerais MG Brazil, C1-1

journal

Funded Projects at Deakin

Other Public Sector Funding

Stress response ofblack bream during recreational catch and release

Dr Paul Jones, A/Prof Luis Afonso

  • 2013: $15,943

Industry and Other Funding

Application and development of biochemical assays to assess smoltification in Atlantic salmon (Salmo salar)

A/Prof Luis Afonso

  • 2014: $18,400

Effects of chronic exposure to different thermal regimens on Atlantic salmon (Salmo salar) growth, stress physiology and health

A/Prof Luis Afonso, Linda Sams, Dr Christine Huynh

  • 2015: $23,000
  • 2014: $24,192

Physiological and molecular indicators to assess smoltification in Atlantic salmon (Salmo salar)

A/Prof Luis Afonso, Dr Paul Jones, Linda Sams

  • 2017: $31,661
  • 2016: $77,838
  • 2015: $40,000

Supervisions

Associate Supervisor
2016

Michael Salini

Thesis entitled: Fatty acid metabolism in barramundi (Lates calcarifer)

Doctor of Philosophy (Life & Env), School of Life and Environmental Sciences